Acid Water-ground Nano-realgar Is Superior to Crude Realgar in Promoting Apoptosis of MCF-7 Breast Cancer Cells.

Szczegóły
Abstrakt

Tytuł:: Acid Water-ground Nano-realgar Is Superior to Crude Realgar in Promoting Apoptosis of MCF-7 Breast Cancer Cells.
Autorzy:: Xi J; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Fang JH; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Xiong XM; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Gui C; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Wang YX; School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 430065, China. .
Zhang XQ; School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China. .
Źródło:: Current medical science [Curr Med Sci] 2022 Aug; Vol. 42 (4), pp. 720-732. Date of Electronic Publication: 2022 Jul 05.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Wuhan : Huazhong University of Science and Technology, [2018]-
MeSH Terms:: Breast Neoplasms*/drug therapy
Apoptosis ; Cell Line, Tumor ; Female ; Humans ; MCF-7 Cells ; Water/pharmacology
References:: Sung H, Ferlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin, 2021,71(3):209–249. (PMID: 33538338)
Jemal A, Siegel R, Xu J, et al. Cancer statistics, 2010. CA Cancer J Clin, 2010,60(5):277–300. (PMID: 2061054310.3322/caac.20073)
Niu QY, Chen WP, Tian YZ, et al. Preparation of medicinal realgar using realgar tailings. Chem Environ Protect (Chinese), 2002,22(3):169–172.
Yao CM, Xiong XX, Liu CT. Metallogenic Fluid Evolution and Mineralization of Shimen Realgar Deposit, Hunan. Deposit Geol (Chinese), 1998,17(26): 1063–1066.
Niu M, Shen Y, Qi J, et al. Effects of realgar (As 4 S 4 ) on degradation of PML-RARA harboring acquired arsenic-resistance mutations. Ann Hematol, 2017,96:1945–1948. (PMID: 2882193310.1007/s00277-017-3093-8)
Wang L, Zhou GB, Liu P, et al. Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia. Proc Natl Acad Sci USA, 2008,105:4826–4831. (PMID: 18344322229078410.1073/pnas.0712365105)
Liu Y, He P, Cheng X, et al. Long-term outcome of 31 cases of refractory acute promyelocytic leukemia treated with compound realgar natural indigo tablets administered alternately with chemotherapy. Oncol Lett, 2015,10:1184–1190. (PMID: 26622649450914210.3892/ol.2015.3308)
Mao JH, Sun XY, Liu JX, et al. As 4 S 4 targets RING-type E3 ligase c-CBL to induce degradation of BCR-ABL in chronic myelogenous leukemia. Proc Natl Acad Sci USA, 2010,107:21683–21688. (PMID: 21118980300302010.1073/pnas.1016311108)
Yin T, Wu YL, Sun HP, et al. Combined effects of As 4 S 4 and imatinib on chronic myeloid leukemia cells and BCR-ABL oncoprotein. Blood, 2004,104:4219–4225. (PMID: 1533985210.1182/blood-2004-04-1433)
Wu JZ, Ho PC. Evaluation of the in vitro activity and in vivo bioavailability of realgar nanoparticles prepared by cryo-grinding. Eur J Pharm Sci, 2006,29:35–44. (PMID: 1682473910.1016/j.ejps.2006.05.002)
Liao Q, Ji L, Deng F, et al. Study on the variation rule of As 2 S 2 and As 2 O 3 content in different realgar processed products. Tradition Chin Med Clin (Chinese), 2013,4(2):21–24.
Guo T, Shi F, Yang G, et al. Effect of solid dispersion technology on stability and in vitro dissolution of realgar nanoparticles. Chin J Tradit Chin Med (Chinese), 2013,38(17):2782–2787.
Tian Y, Wang X, Xi R, et al. Enhanced antitumour activity of realgar mediated by milling it to nano-size. Int J Nanomed, 2014,9:745–757.
Wang XB, Gao HY, Hou BL, et al. Nanoparticle realgar powders induce apoptosis in U937 cells through caspase MAPK and mitochondrial pathways. Arch Pharm Res, 2007,30(5):653–658. (PMID: 1761568710.1007/BF02977662)
Wang YS, Zhou ST, Wei HL. Apoptosis effects of drug sensitivity leukemia cells induced by nano-realgar. China J Chin Materia Medica (Chinese), 2013,38(13):2202–2205.
Wang XB, Xian RG, Shi Y, et al. Optimization study of nano-realgar preparation process. J Chin Pharm Sci of PLA (Chinese), 2008,24(06):471–474.
Shi F, Feng NP, Omari-Siaw E. Realgar nanoparticle-based microcapsules: preparation and in-vitro/in-vitro characterizations. J Pharm Pharmacol, 2015,67(1):35–42. (PMID: 2519657210.1111/jphp.12314)
Zhao Y, Yuan B, Onda K, et al. Anticancer efficacies of arsenic disulfide through apoptosis induction, cell cycle arrest, and pro-survival signal inhibition in human breast cancer cells. Am J Cancer Res, 2018,8(3):366–386. (PMID: 296369955883090)
Zhao Y, Onda K, Sugiyama K, et al. Antitumour effects of arsenic disulfide on the viability, migratory ability, apoptosis and autophagy of breast cancer cells. Oncol Rep, 2019,41(1):27–42. (PMID: 30320388)
Khairul I, Wang QQ, Jiang YH, et al. Metabolism, toxicity and anticancer activities of arsenic compounds. Oncotarget, 2017,8(14):23905–23926. (PMID: 28108741541035410.18632/oncotarget.14733)
Wu J, Shao Y, Liu J, et al. The medicinal use of realgar (As 4 S 4 ) and its recent development as an anticancer agent. J Ethnopharmacol, 2011,135(3):595–602. (PMID: 2149764910.1016/j.jep.2011.03.071)
Liu X, Li X, Wang L, et al. Realgar induces apoptosis in the chronic lymphocytic leukemia cell line MEC-1. Mol Med Rep, 2013,8(6):1866–1870. (PMID: 2414172710.3892/mmr.2013.1731)
Yang MH, Wan WQ, Luo JS, et al. Multicenter randomized trial of arsenic trioxide and Realgar-Indigo naturalis formula in pediatric patients with acute promyelocytic leukemia: Interim results of the SCCLG-APL clinical study. Am J Hematol, 2018,93(12):1467–1473. (PMID: 30160789628284710.1002/ajh.25271)
Zhao QH, Zhang Y, Liu Y, et al. Anticancer effect of realgar nanoparticles on mouse melanoma skin cancer in vivo via transdermal drug delivery. Med Oncol, 2010,27(2):203–212. (PMID: 1928037210.1007/s12032-009-9192-1)
Qi YF, Li HJ, Liu ZD. The effect of nano-realgar on the proliferation of lung cancer A549 cells and its mechanism. Chin J Cancer Prevent Treat (Chinese), 2013,20(01):27–30.
Song P, Chen P, Wang D, et al. Realgar transforming solution displays anticancer potential against human hepatocellular carcinoma HepG2 cells by inducing ROS. Int J Oncol, 2017,50(2):660–670. (PMID: 2803541810.3892/ijo.2016.3831)
Zhang MH, Chen JQ, Guo HM, et al. Combination of LC/MS and GC/MS based metabolomics to study the hepatotoxic effect of realgar nanoparticles in rats. Chin J Nat Med, 2017,15(9):684–694. (PMID: 28991530)
Wang G, Zhang T, Sun W, et al. Arsenic sulfide induces apoptosis and autophagy through the activation of ROS/JNK and suppression of Akt/mTOR signaling pathways in osteosarcoma. Free Radic Biol Med, 2017,106:24–37. (PMID: 2818892310.1016/j.freeradbiomed.2017.02.015)
Xi XX, Sun J, Xi DB, et al. Realgar Nanoparticles Inhibit Migration, Invasion and Metastasis in a Mouse Model of Breast Cancer by Suppressing Matrix Metalloproteinases and Angiogenesis. Curr Drug Deliv, 2020,17(2):148–158. (PMID: 10.2174/1567201817666200115105633)
An YL, Nie F, Wang ZY, et al. Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells. Int J Nanomed, 2011,6:3187–3194. (PMID: 10.2147/IJN.S26237)
Qin YU, Wang H, Liu ZY, et al. Realgar quantum dots induce apoptosis and necrosis in HepG2 cells through endoplasmic reticulum stress. Biomed Rep, 2015,3(5):657–662. (PMID: 26405541453483210.3892/br.2015.489)
Ding W, Zhang L, Kim S, et al. Arsenic sulfide as a potential anti-cancer drug. Mol Med Rep, 2015,11:968–974. (PMID: 2537126510.3892/mmr.2014.2838)
Tse WP, Cheng CH, Che CT, et al. Arsenic trioxide, arsenic pentoxide, and arsenic iodide inhibit human keratinocyte proliferation through the induction of apoptosis. J Pharmacol Exp Ther, 2008,326(2):388–94. (PMID: 1845131810.1124/jpet.107.134080)
Zhao WZ, Lu X, Yuan Y, et al. Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines. Int J Nanomed, 2011,6:1569–1577. (PMID: 10.2147/IJN.S21373)
Wang Y, Zhang Y, Yang L, et al. Arsenic trioxide induces the apoptosis of human breast cancer MCF-7 cells through activation of caspase-3 and inhibition of HERG channels. Exp Ther Med, 2011,2(3):481–486. (PMID: 22977528344070210.3892/etm.2011.224)
Shi Y, Cao T, Huang H, Lian CQ, et al. Arsenic trioxide inhibits cell growth and motility via up-regulation of let-7a in breast cancer cells. Cell Cycle, 2017,16(24): 2396–2403. (PMID: 28980872578843210.1080/15384101.2017.1387699)
Cheng YX, Liu R, Wang Q, et al. Realgar-induced apoptosis of cervical cancer cell line Siha via cytochrome c release and caspase-3 and caspase-9 activation. Chin J Inter Med, 2012,18:359–365. (PMID: 10.1007/s11655-011-0697-z)
Wang XB, Gao HY, Hou BL, et al. Nanoparticle realgar powders induce apoptosis in U937 cells through caspase MAPK and mitochondrial pathways. Arch Pharm Res, 2007,30:653–658. (PMID: 1761568710.1007/BF02977662)
Liu R, Pu D, Liu Y, et al. Induction of SiHa cells apoptosis by nanometer realgar suspension and its mechanism. J Huazhong Univ Sci Technolog Med Sci, 2008,28(3):317–321. (PMID: 1856333210.1007/s11596-008-0320-5)
Wang S, He MF, Li LM, et al. Cell-in-Cell Death Is Not Restricted by Caspase-3 Deficiency in MCF-7 Cells. J Breast Cancer, 2016,19(3):231–241. (PMID: 27721872505330710.4048/jbc.2016.19.3.231)
Changizi V, Azariasl S, Motevaseli E, et al. Assessment Synergistic Effects of Integrated Therapy with Epigallocatechin-3-Gallate (EGCG) & Arsenic Trioxide and Irradiation on Breast Cancer Cell Line. Iran J Public Health, 2020,49(8):1555–1563. (PMID: 330833337554386)
Gao H, Zhang Y, Dong L, et al. Triptolide induces autophagy and apoptosis through ERK activation in human breast cancer MCF-7 cells. Exp Ther Med (Chinese), 2018,15(4):3413–3419.
Contributed Indexing:: Keywords: MCF-7 cells; acid water-ground nano-realgar; apoptosis; realgar
Substance Nomenclature:: 059QF0KO0R (Water)
Entry Date(s):: Date Created: 20220705 Date Completed: 20220816 Latest Revision: 20220816
Update Code:: 20240105
DOI:: 10.1007/s11596-022-2605-5
PMID:: 35788945
: Czasopismo naukowe

Full Text Finder

Objective: Realgar is a traditional mineral Chinese medicine with antitumor effects, but it has high toxicity and low efficacy in its crude form. The purpose of this study was to optimize realgar to increase its efficacy and therapeutic potential.
Methods: Crude realgar (CR) was mechanically ground to obtain nano-realgar (NR), and then nano-realgar processed products (NRPPs) were obtained using three different traditional Chinese medicine processing methods: grinding in water, acid water, and alkali water, respectively.
Results: By analyzing the size distribution of nanoparticles and the content of arsenic trioxide (As 2 O 3 ; ATO), we found that acid water-ground NRPPs had the characteristics of high purity and low toxicity. The effects of CR, NR, and NRPPs on proliferation, cell cycle, and apoptosis of MCF-7 cells were detected, and the ability of NRPPs to induce apoptosis in MCF-7 cells was analyzed. The results showed that the average particle size of acid water-ground NRPPs was 137.7 nm, and the content of ATO was 2.83 mg/g. Acid water-ground NRPPs showed better effects on inhibiting proliferation, cell cycle, and apoptosis of MCF-7 cells than CR and NR. Western blot assays further confirmed that acid water-ground NRPPs upregulated the protein expression of TP53, Bax, cytochrome c, caspase-9, and caspase-3 in MCF-7 cells (P<0.05) and inhibited the expression of Bcl-2 (P<0.05).
Conclusion: These results suggest that acid water-ground NRPPs can induce apoptosis of MCF-7 cells through regulating mitochondrial-mediated apoptosis, providing evidence for the clinical application of realgar.
(© 2022. Huazhong University of Science and Technology.)

Informacja