Larva migrans in BALB/c mice experimentally infected with Toxocara cati ensured by PCR assay.

Tytuł:: Larva migrans in BALB/c mice experimentally infected with Toxocara cati ensured by PCR assay.
Autorzy:: Naderbandi M; Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Zibaei M; Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, P.O. Box: 3149779453, Karaj, Iran. .
Haniloo A; Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. .
Firoozeh F; Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Hatami Z; Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, P.O. Box: 3149779453, Karaj, Iran.
Shokri E; Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Taira K; Laboratory of Parasitology, School of Veterinary Medicine, Azabu University, Fuchinobe, Sagamihara, Kanagawa, Japan.
Źródło:: BMC veterinary research [BMC Vet Res] 2022 Jul 05; Vol. 18 (1), pp. 257. Date of Electronic Publication: 2022 Jul 05.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central, 2005-
MeSH Terms:: Larva Migrans*/veterinary
Toxocariasis*/parasitology
Animals ; Larva ; Mice ; Mice, Inbred BALB C ; Ovum ; Pepsin A ; Polymerase Chain Reaction/veterinary ; Toxocara
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Contributed Indexing:: Keywords: BALB/c mice; Larva Migrans; Microscopic larvae count; PCR; Toxocara cati
Substance Nomenclature:: EC 3.4.23.1 (Pepsin A)
Entry Date(s):: Date Created: 20220705 Date Completed: 20220707 Latest Revision: 20240102
Update Code:: 20240102
PubMed Central ID:: PMC9254581
DOI:: 10.1186/s12917-022-03366-6
PMID:: 35791007
: Czasopismo naukowe

Pełny tekst

Background: Toxocara cati, the cat roundworm, is a parasitic nematode that known to cause toxocariasis in intermediate hosts and humans. In this study, we characterized the dynamics of T. cati larvae migration in BALB/c mice after inoculation with eggs and ensured the migration detecting the larval DNA by a PCR. To evaluate the dynamics of larval migration and distribution, twenty-four BALB/c mice were orally inoculated with 2500 T. cati infective eggs and the visceral organs of the infected animals were examined by pepsin digestion and microscopic parasite counts, followed by PCR at day 1 to 28 post-inoculation.
Results: The PCR assays were successfully used for detection of T. cati larvae in tissue samples and T. cati larvae and the DNAs were found in the liver, lungs, heart, kidneys and the brain. We detected T. cati in 92.2% of tissue samples by PCR, 30% higher than the conventional pepsin digestion technique.
Conclusion: Our findings demonstrated that the PCR assay is a sensitive and specific for the detection of T. cati larvae. Therefore, it could become a useful tool for the investigation of the dynamics of larval migration and Toxocara infection in murine model.
(© 2022. The Author(s).)

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