Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection.

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Tytuł:: Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection.
Autorzy:: Zhang M; Guizhou University, School of Medicine, Guiyang, China.
Du Y; Center for Disease Control and Prevention of Shanxi Province, Xian, China.
Yang L; Guizhou University, School of Medicine, Guiyang, China; Guizhou Provincial People's Hospital, Guiyang, China.
Zhan L; Guizhou Provincial People's Hospital, Guiyang, China; NHC Key Laboratory of Pulmonary Immunological Diseases, Guizhou Provincial People's Hospital, Guiyang, China.
Yang B; Guizhou Provincial People's Hospital, Guiyang, China; NHC Key Laboratory of Pulmonary Immunological Diseases, Guizhou Provincial People's Hospital, Guiyang, China.
Huang X; Center for Disease Control and Prevention of Henan Province, Zhengzhou, China.
Xu B; Center for Disease Control and Prevention of Henan Province, Zhengzhou, China.
Morita K; Nagasaki University, Institute of Tropical Medicine, Department of Virology, Nagasaki, Japan.
Yu F; Guizhou Provincial People's Hospital, Guiyang, China; NHC Key Laboratory of Pulmonary Immunological Diseases, Guizhou Provincial People's Hospital, Guiyang, China. Electronic address: .
Źródło:: The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases [Braz J Infect Dis] 2022 Jul-Aug; Vol. 26 (4), pp. 102386. Date of Electronic Publication: 2022 Jul 11.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2010- : Rio de Janeiro : Elsevier Editora
Original Publication: Salvador, Bahia, Brazil : Contexto, c1997-
MeSH Terms:: Enzyme-Linked Immunosorbent Assay*/methods
Phlebovirus*
Severe Fever with Thrombocytopenia Syndrome*/diagnosis
Animals ; Antibodies, Monoclonal ; Antibodies, Viral ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Mice ; Mice, Inbred BALB C ; Nucleocapsid Proteins ; Recombinant Proteins
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Contributed Indexing:: Keywords: Monoclonal antibody; Recombinant nucleocapsid protein; Severe fever with thrombocytopenia syndrome virus
Substance Nomenclature:: 0 (Antibodies, Monoclonal)
0 (Antibodies, Viral)
0 (Immunoglobulin G)
0 (Immunoglobulin M)
0 (Nucleocapsid Proteins)
0 (Recombinant Proteins)
Entry Date(s):: Date Created: 20220714 Date Completed: 20220831 Latest Revision: 20220912
Update Code:: 20240104
PubMed Central ID:: PMC9459026
DOI:: 10.1016/j.bjid.2022.102386
PMID:: 35835158
: Czasopismo naukowe

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Introduction: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerged virus that poses a great threat to human health because of high fatality rate.
Methods: To develop sensitive and specific sero-diagnostic systems for SFTSV infections, monoclonal antibodies (MAbs) against recombinant SFTSV nucleocapsid (rSFTSV-N) protein were developed by immunizing BALB/C mice with rSFTSV-N protein and fusing the spleen cells with SP2/0 myeloma cells. Three hybridoma cell lines secreting MAbs against rSFTSV-N were obtained. MAb based IgG sandwich enzyme linked immunosorbent assay (ELISA) and IgM capture ELISA systems were established by using the newly developed MAbs. One hundred fifteen clinical suspected SFTS patients serum samples were used to evaluate the newly established systems by comparing with the total antibody detecting sandwich ELISA system and indirect ELISA systems.
Results: The MAbs based sandwich IgG ELISA was perfectly matched with that of the total antibody sandwich ELISA and the indirect IgG ELISA. IgM capture ELISA results perfectly matched with that of the total antibody sandwich ELISA while detecting eight additional positive samples missed by the indirect IgM ELISA.
Conclusions: The MAbs against rSFTSV-N protein offer new tools for SFTSV studies and our newly developed MAb-based IgG and IgM capture ELISA systems would offer safe and useful tools for diagnosis of SFTS virus infections and epidemiological investigations.
Competing Interests: Conflicts of interest The authors declare no conflicts of interest.
(Copyright © 2022 Sociedade Brasileira de Infectologia. Published by Elsevier España, S.L.U. All rights reserved.)

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