Resistance exercise with different workloads have distinct effects on cellular respiration of peripheral blood mononuclear cells.

Tytuł:: Resistance exercise with different workloads have distinct effects on cellular respiration of peripheral blood mononuclear cells.
Autorzy:: Lähteenmäki EI; Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.; Research Centre for Physical Activity and Health LIKES, Jyväskylä, Finland.
Koski M; Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.; Biology of Physical Activity, Neuromuscular Research Center, University of Jyväskylä, Jyväskylä, Finland.
Koskela I; Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.
Lehtonen E; Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.
Kankaanpää A; Gerontology Research Center (GEREC), Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.
Kainulainen H; Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.
Walker S; Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.; Biology of Physical Activity, Neuromuscular Research Center, University of Jyväskylä, Jyväskylä, Finland.
Lehti M; Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.; Research Centre for Physical Activity and Health LIKES, Jyväskylä, Finland.
Źródło:: Physiological reports [Physiol Rep] 2022 Jul; Vol. 10 (14), pp. e15394.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013-
MeSH Terms:: Leukocytes, Mononuclear*/metabolism
Resistance Training*
C-Reactive Protein/metabolism ; Cell Respiration ; Exercise/physiology ; Humans ; Inflammation/metabolism ; Lactic Acid ; Leukocytes/metabolism ; Male ; Muscle, Skeletal/metabolism ; Workload
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Contributed Indexing:: Keywords: bioenergetics; mitochondria; resistance training; training volume; white blood cells
Substance Nomenclature:: 33X04XA5AT (Lactic Acid)
9007-41-4 (C-Reactive Protein)
Entry Date(s):: Date Created: 20220719 Date Completed: 20220721 Latest Revision: 20231211
Update Code:: 20240104
PubMed Central ID:: PMC9295128
DOI:: 10.14814/phy2.15394
PMID:: 35852047
: Czasopismo naukowe

Pełny tekst

Little is known how acute exercise-induced inflammation and metabolic stress affect immune cell bioenergetics and the portion of its components. Therefore, we investigated acute effects of eccentric-only (E), concentric-only (C) and combined eccentric-concentric resistance exercise (E + C) bouts on cellular respiration of peripheral blood mononuclear cells (PBMCs). Twelve strength-trained young men performed bench press resistance exercises in randomized order. Venous blood samples were drawn at pre-, 5 min post- and 24 h post-exercise. Several PBMC respiration states were measured using high-resolution respirometry. Levels of leukocytes, interleukin 6 (IL-6), C-reactive protein (CRP), creatine kinase (CK), blood lactate and maximum voluntary isometric force were measured from the same time points. Effects of blood lactate and pH change on bioenergetics of PBMCs were investigated ex vivo. PBMC routine respiration (p = 0.017), free routine capacity (p = 0.025) and ET-capacity (p = 0.038) decreased immediately after E + C. E responded in opposite manner 5 min post-exercise compared to E + C (p = 0.013) and C (p = 0.032) in routine respiration, and to E + C in free routine activity (p = 0.013). E + C > C > E was observed for increased lactate levels and decreased isometric force that correlated with routine respiration (R = -0.369, p = 0.035; R = 0.352, p = 0.048). Lactate and pH change did not affect bioenergetics of PBMCs. Acute resistance exercise affected cellular respiration of PBMCs, with training volume and the amount of metabolic stress appear influential. Results suggest that acute inflammation response does not contribute to changes seen in cellular respiration, but the level of peripheral muscle fatigue and metabolic stress could be explaining factors.
(© 2022 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)

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