A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication.

Tytuł:: A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication.
Autorzy:: Kozhukhar N; Department of Physiology and Cell Biology, University of South Alabama, Mobile, AL 36688, USA.
Spadafora D; Department of Physiology and Cell Biology, University of South Alabama, Mobile, AL 36688, USA.
Rodriguez YAR; Department of Physiology and Cell Biology, University of South Alabama, Mobile, AL 36688, USA.
Alexeyev MF; Department of Physiology and Cell Biology, University of South Alabama, Mobile, AL 36688, USA.
Źródło:: Cells [Cells] 2022 Jul 11; Vol. 11 (14). Date of Electronic Publication: 2022 Jul 11.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI
MeSH Terms:: DNA-Binding Proteins*/genetics
DNA-Binding Proteins*/metabolism
Transcription Factors*/genetics
Transcription Factors*/metabolism
DNA, Mitochondrial/genetics ; Humans ; Mitochondrial Proteins/genetics ; Mitochondrial Proteins/metabolism ; Reverse Genetics
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Grant Information:: S10 OD025089 United States OD NIH HHS; R01 OD010944 United States OD NIH HHS; P01 HL066299 United States HL NHLBI NIH HHS
Contributed Indexing:: Keywords: GeneSwap approach; TFAM; TFAM chimeras; TFAM knockout; TFAM-mtDNA evolutionary co-adaptation; mtDNA instability; mtDNA metabolism; mtDNA replication; mtDNA transcription
Substance Nomenclature:: 0 (DNA, Mitochondrial)
0 (DNA-Binding Proteins)
0 (Mitochondrial Proteins)
0 (Transcription Factors)
Entry Date(s):: Date Created: 20220727 Date Completed: 20220728 Latest Revision: 20221106
Update Code:: 20240105
PubMed Central ID:: PMC9316749
DOI:: 10.3390/cells11142168
PMID:: 35883613
: Czasopismo naukowe

Pełny tekst

The unavailability of tractable reverse genetic analysis approaches represents an obstacle to a better understanding of mitochondrial DNA replication. Here, we used CRISPR-Cas9 mediated gene editing to establish the conditional viability of knockouts in the key proteins involved in mtDNA replication. This observation prompted us to develop a set of tools for reverse genetic analysis in situ, which we called the GeneSwap approach. The technique was validated by identifying 730 amino acid (aa) substitutions in the mature human TFAM that are conditionally permissive for mtDNA replication. We established that HMG domains of TFAM are functionally independent, which opens opportunities for engineering chimeric TFAMs with customized properties for studies on mtDNA replication, mitochondrial transcription, and respiratory chain function. Finally, we present evidence that the HMG2 domain plays the leading role in TFAM species-specificity, thus indicating a potential pathway for TFAM-mtDNA evolutionary co-adaptations.

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