3-Bromopyruvate, a caloric restriction mimetic, exerts a mitohormetic effect to provide neuroprotection through activation of autophagy in rats during aging.

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Tytuł:: 3-Bromopyruvate, a caloric restriction mimetic, exerts a mitohormetic effect to provide neuroprotection through activation of autophagy in rats during aging.
Autorzy:: Arya JK; Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh, 211002, India.
Kumar R; Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh, 211002, India.
Tripathi SS; Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh, 211002, India.
Rizvi SI; Department of Biochemistry, University of Allahabad, Allahabad, Uttar Pradesh, 211002, India. .
Źródło:: Biogerontology [Biogerontology] 2022 Oct; Vol. 23 (5), pp. 641-652. Date of Electronic Publication: 2022 Sep 01.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Dordrecht ; Boston : Kluwer Academic, c2000-
MeSH Terms:: Caloric Restriction*
Neuroprotection*
Aging/metabolism ; Animals ; Antioxidants/metabolism ; Antioxidants/pharmacology ; Autophagy ; Biomarkers/metabolism ; Male ; Oxidative Stress ; Pyruvates ; Rats ; Reactive Oxygen Species/metabolism
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Contributed Indexing:: Keywords: 3-Bromopyruvate; Autophagy; Mitohormesis; Neuroprotection; Reactive oxygen species
Substance Nomenclature:: 0 (Antioxidants)
0 (Biomarkers)
0 (Pyruvates)
0 (Reactive Oxygen Species)
63JMV04GRK (bromopyruvate)
Entry Date(s):: Date Created: 20220901 Date Completed: 20220915 Latest Revision: 20220923
Update Code:: 20240105
DOI:: 10.1007/s10522-022-09988-5
PMID:: 36048311
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In the present study, attempts have been made to evaluate the potential role of 3 Bromopyruvate (3-BP) a glycolytic inhibitor and a caloric restriction mimetic (CRM), to exert neuroprotection in rats during aging through modulation of autophagy. Young male rats (4 months), and naturally aged (22 months) male rats were supplemented with 3-BP (30 mg/kg b.w., orally) for 28 days. Our results demonstrate a significant increase in the antioxidant biomarkers (ferric reducing antioxidant potential level, total thiol, superoxide dismutase, and catalase activities) and a decrease in the level of pro-oxidant biomarkers such as protein carbonyl after 3-BP supplementation in brain tissues. A significant increase in reactive oxygen species (ROS) was observed due to the mitohormetic effect of 3-BP supplementation in the treated rats. Furthermore, the 3-BP treatment also enhanced the activities of electron transport chain complexes I and IV in aged brain mitochondria thus proving its antioxidant potential at the level of mitochondria. Gene expression analysis with reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to assess the expression of autophagy, neuroprotective and aging marker genes. RT-PCR data revealed that 3-BP up-regulated the expression of autophagy markers genes (Beclin-1 and LC3 β), sirtuin-1, and neuronal marker gene (NSE), respectively in the aging brain. The results suggest that 3-BP induces a mitohormetic effect through the elevation of ROS which reinforces defensive mechanism(s) targeted at regulating autophagy. These findings suggest that consistently low-dose 3-BP may be beneficial for neuroprotection during aging and age-related disorders.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)

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