RNY3 modulates cell proliferation and IL13 mRNA levels in a T lymphocyte model: a possible new epigenetic mechanism of IL-13 regulation.

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Tytuł:: RNY3 modulates cell proliferation and IL13 mRNA levels in a T lymphocyte model: a possible new epigenetic mechanism of IL-13 regulation.
Autorzy:: Estravís M; Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.; Red Cooperativa de Investigación en Salud-RETICS ARADyAL, ISCIII, Madrid, Spain.
García-Sánchez A; Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain. .; Red Cooperativa de Investigación en Salud-RETICS ARADyAL, ISCIII, Madrid, Spain. .; Departamento de Ciencias Biomédicas y del Diagnóstico, Universidad de Salamanca, Salamanca, Spain. .
Martin MJ; Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.; Red Cooperativa de Investigación en Salud-RETICS ARADyAL, ISCIII, Madrid, Spain.; Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Salamanca, Spain.
Pérez-Pazos J; Unidad de Farmacogenética y Medicina de Precisión, Servicio de Bioquímica Clínica, Servicio de Alergología, Hospital Universitario de Salamanca, IBSAL, Salamanca, Spain.
Isidoro-García M; Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.; Red Cooperativa de Investigación en Salud-RETICS ARADyAL, ISCIII, Madrid, Spain.; Servicio de Bioquímica Clínica, Complejo Asistencial Universitario de Salamanca, Salamanca, Spain.; Departamento de Medicina, Universidad de Salamanca, Salamanca, Spain.
Dávila I; Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.; Red Cooperativa de Investigación en Salud-RETICS ARADyAL, ISCIII, Madrid, Spain.; Departamento de Ciencias Biomédicas y del Diagnóstico, Universidad de Salamanca, Salamanca, Spain.; Servicio de Inmunoalergia, Complejo Asistencial Universitario de Salamanca, Salamanca, Spain.
Sanz C; Instituto de Investigación Biomédica de Salamanca (IBSAL), Salamanca, Spain.; Red Cooperativa de Investigación en Salud-RETICS ARADyAL, ISCIII, Madrid, Spain.; Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Źródło:: Journal of physiology and biochemistry [J Physiol Biochem] 2023 Feb; Vol. 79 (1), pp. 59-69. Date of Electronic Publication: 2022 Sep 12.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Pamplona, Spain : Servicio de Publicaciones de la Universidad De Navarra (departamentos de fisiología y nutrición y de bioquimica), [1998-
MeSH Terms:: Asthma*
Interleukin-13*/metabolism
RNA, Untranslated*/metabolism
Humans ; Cell Proliferation ; Epigenesis, Genetic ; Lymphocyte Activation ; RNA, Messenger ; T-Lymphocytes ; Tetradecanoylphorbol Acetate/pharmacology
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Grant Information:: PI17/00818 Instituto de Salud Carlos III; PI20/00268 Instituto de Salud Carlos III; RD16/0006/0019 Instituto de Salud Carlos III; IES161P20 Consejería de Educación, Junta de Castilla y León; GRS2156/A/20 Gerencia Regional de Salud, Junta de Castilla y León
Contributed Indexing:: Keywords: Epigenetics; Gene expression; Interleukin 13 (IL-13); Jurkat cells; Lymphocyte activation; Y-RNA
Substance Nomenclature:: 0 (Interleukin-13)
0 (RNA, Messenger)
NI40JAQ945 (Tetradecanoylphorbol Acetate)
0 (RNA, Untranslated)
Entry Date(s):: Date Created: 20220911 Date Completed: 20230217 Latest Revision: 20230217
Update Code:: 20240104
PubMed Central ID:: PMC9905197
DOI:: 10.1007/s13105-022-00920-6
PMID:: 36089628
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Allergic asthma is the most common type of asthma. It is characterized by T H 2 cell-driven inflammation in which interleukin-13 (IL-13) plays a pivotal role. Cytoplasmic RNAs (Y-RNAs), a variety of non-coding RNAs that are dysregulated in many cancer types, are also differentially expressed in patients with allergic asthma. Their function in the development of the disease is still unknown. We investigated the potential role of RNY3 RNA (hY3) in the T H 2 cell inflammatory response using the Jurkat cell line as a model. hY3 expression levels were modulated to mimic the upregulation effect in allergic disease. We evaluated the effect of hY3 over cell stimulation and the expression of the T H 2 cytokine IL13. Total RNA was isolated and retrotranscribed, and RNA levels were assessed by qPCR. In Jurkat cells, hY3 levels increased upon stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. When transfecting with high levels of hY3 mimic molecules, cell proliferation rate decreased while IL13 mRNA levels increased upon stimulation compared to stimulated control cells. Our results show the effect of increased hY3 levels on cell proliferation and the levels of IL13 mRNA in Jurkat cells. Also, we showed that hY3 could act over other cells via exosomes. This study opens up new ways to study the potential regulatory function of hY3 over IL-13 production and its implications for asthma development.
(© 2022. The Author(s).)

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