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Tytuł:
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No cell is an island: characterising the leaf epidermis using epidermalmorph, a new R package.
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Autorzy:
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Brown MJM; Biological Sciences, University of Tasmania, Hobart, 7000, Tas., Australia.; Royal Botanic Gardens Kew, Richmond, TW9 3AE, UK.
Jordan GJ; Biological Sciences, University of Tasmania, Hobart, 7000, Tas., Australia.
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Źródło:
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The New phytologist [New Phytol] 2023 Jan; Vol. 237 (1), pp. 354-366. Date of Electronic Publication: 2022 Nov 15.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: Oxford : Wiley on behalf of New Phytologist Trust
Original Publication: London, New York [etc.] Academic Press.
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MeSH Terms:
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Plant Stomata*/physiology
Plant Leaves*/physiology
Reproducibility of Results ; Epidermal Cells ; Plants ; Epidermis ; Plant Epidermis/physiology
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References:
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Contributed Indexing:
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Keywords: R package; cell arrangement; cell shape; epidermal cells; stomatal morphology
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Entry Date(s):
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Date Created: 20221007 Date Completed: 20221207 Latest Revision: 20230415
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Update Code:
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20240105
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PubMed Central ID:
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PMC10098627
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DOI:
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10.1111/nph.18519
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PMID:
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36205061
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The leaf epidermis is the interface between a plant and its environment. The epidermis is highly variable in morphology, with links to both phylogeny and environment, and this diversity is relevant to several fields, including physiology, functional traits, palaeobotany, taxonomy and developmental biology. Describing and measuring leaf epidermal traits remains challenging. Current approaches are either extremely labour-intensive and not feasible for large studies or limited to measurements of individual cells. Here, we present a method to characterise individual cell size, shape (including the effect of neighbouring cells) and arrangement from light microscope images. We provide the first automated characterisation of cell arrangement (from traced images) as well as multiple new shape characteristics. We have implemented this method in an R package, epidermalmorph, and provide an example workflow using this package, which includes functions to evaluate trait reliability and optimal sampling effort for any given group of plants. We demonstrate that our new metrics of cell shape are independent of gross cell shape, unlike existing metrics. epidermalmorph provides a broadly applicable method for quantifying epidermal traits that we hope can be used to disentangle the fundamental relationships between form and function in the leaf epidermis.
(© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)