Effects of Age and Lifelong Moderate-Intensity Exercise Training on Rats' Testicular Function.

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Abstrakt

Tytuł:: Effects of Age and Lifelong Moderate-Intensity Exercise Training on Rats' Testicular Function.
Autorzy:: Silva JV; Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.; QOPNA & LAQV, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.; Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal.
Santiago J; Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
Matos B; Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
Henriques MC; Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
Patrício D; Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
Martins AD; Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal.
Duarte JA; Research Center in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, 4200-450 Porto, Portugal.
Ferreira R; QOPNA & LAQV, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Alves MG; Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal.
Oliveira P; Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal.
Oliveira PF; QOPNA & LAQV, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Fardilha M; Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2022 Oct 01; Vol. 23 (19). Date of Electronic Publication: 2022 Oct 01.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:: Age Factors*
Physical Conditioning, Animal*/physiology
Testis*/physiology
Animals ; Antioxidants/metabolism ; Atrophy ; HSP27 Heat-Shock Proteins ; Male ; Nuclear Respiratory Factor 1 ; RNA, Messenger/metabolism ; Rats ; Rats, Wistar ; Semen/metabolism ; Transcription Factors
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Grant Information:: PTDC/DTP-DES/6077/2014 Fundação para a Ciência e Tecnologia; UIDB/04501/2020 Institute for Biomedicine; PEst-OE/SAU/UI0215/2019 UMIB; UID/QUI/00062/2019 QOPNA; UIDB/04033/2020 Fundação para a Ciência e Tecnologia; SFRH/BPD/123155/2016 Fundação para a Ciência e Tecnologia; SFRH/BD/136896/2018 Fundação para a Ciência e Tecnologia
Contributed Indexing:: Keywords: aging; mitochondrial function; physical exercise; protein synthesis; rodent model; stress response; testicular atrophy
Substance Nomenclature:: 0 (Antioxidants)
0 (HSP27 Heat-Shock Proteins)
0 (Nuclear Respiratory Factor 1)
0 (RNA, Messenger)
0 (Transcription Factors)
Entry Date(s):: Date Created: 20221014 Date Completed: 20221017 Latest Revision: 20221019
Update Code:: 20240104
PubMed Central ID:: PMC9570257
DOI:: 10.3390/ijms231911619
PMID:: 36232916
: Czasopismo naukowe

Pełny tekst

Aging is associated with testicular morphological and functional alterations, but the underlying molecular mechanisms and the impact of physical exercise are poorly understood. In this study, we examined the effects of age and lifelong moderate-intensity exercise on rat testis. Mature adults (35 weeks) and middle-aged (61 weeks) Wistar Unilever male rats were maintained as sedentary or subjected to a lifelong moderate-intensity treadmill training protocol. Testis weight and histology, mitochondrial biogenesis and function, and proteins involved in protein synthesis and stress response were evaluated. Our results illustrate an age-induced testicular atrophy that was associated with alterations in stress response, and mitochondrial biogenesis and function. Aging was associated with increased testicular levels of heat shock protein beta-1 (HSP27) and antioxidant enzymes. Aging was also associated with decreased mRNA abundance of the nuclear respiratory factor 1 ( Nrf1 ), a key transcription factor for mitochondrial biogenesis, which was accompanied by decreased protein levels of the oxidative phosphorylation system (OXPHOS) complexes subunits in the testes of older animals. On the other hand, exercise did not protect against age-induced testicular atrophy and led to deleterious effects on sperm morphology. Exercise led to an even more pronounced decrease in the Nrf1 mRNA levels in testes of both age groups and was associated with decreased mRNA abundance of other mitochondrial biogenesis markers and decreased protein levels of OXPHOS complexes subunits. Lifelong moderate-intensity exercise training was also associated with an increase in testicular oxidative stress markers and possibly with reduced translation. Together, our results indicate that exercise did not protect against age-induced testicular atrophy and was not associated with beneficial changes in mitochondria and stress response, further activating mechanisms of protein synthesis inhibition.

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