Integrated analysis of miRNAs and mRNA profiling reveals the potential roles of miRNAs in sheep hair follicle development.

Tytuł:: Integrated analysis of miRNAs and mRNA profiling reveals the potential roles of miRNAs in sheep hair follicle development.
Autorzy:: He J; Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.
Huang X; College of Animal Science, Xinjiang Agricultural University, Urumqi, China.
Zhao B; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Liu G; Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.
Tian Y; Institute of Animal Science, Xinjiang Academy of Animal Sciences, Urumqi, China.
Zhang G; Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.
Wei C; Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China.
Mao J; College of Animal Science, Xinjiang Agricultural University, Urumqi, China.
Tian K; Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China. .
Źródło:: BMC genomics [BMC Genomics] 2022 Oct 22; Vol. 23 (1), pp. 722. Date of Electronic Publication: 2022 Oct 22.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central, [2000-
MeSH Terms:: Gene Expression Profiling*/methods
MicroRNAs*/genetics
MicroRNAs*/metabolism
Animals ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Hair Follicle ; Gene Expression Regulation
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Grant Information:: Nos. CARS-39 Agriculture Research System of China; Nos. CXGC2022E10 Agricultural science and technology innovation project of Shandong Academy of Agricultural Sciences
Contributed Indexing:: Keywords: Dermal fibroblasts; Hair follicle; Merino sheep; Western blot; miRNA–mRNA
Substance Nomenclature:: 0 (RNA, Messenger)
0 (MicroRNAs)
Entry Date(s):: Date Created: 20221023 Date Completed: 20221025 Latest Revision: 20221026
Update Code:: 20240105
PubMed Central ID:: PMC9588206
DOI:: 10.1186/s12864-022-08954-2
PMID:: 36273119
: Czasopismo naukowe

Pełny tekst

Background: Merino sheep exhibit high wool production and excellent wool quality. The fleece of Merino sheep is predominantly composed of wool fibers grown from hair follicles (HFs). The HF is a complex biological system involved in a dynamic process governed by gene regulation, and gene expression is regulated by microRNAs (miRNAs). miRNA inhibits posttranscriptional gene expression by specifically binding to target messenger RNA (mRNA) and plays an important role in regulating gene expression, the cell cycle and biological development sequences. The purpose of this study was to examine mRNA and miRNA binding to identify key miRNAs and target genes related to HF development. This will provide new and important insights into fundamental mechanisms that regulate cellular activity and cell fate decisions within and outside of the skin.
Results: We analyzed miRNA data in skin tissues collected from 18 Merino sheep on four embryonic days (E65, E85, E105 and E135) and two postnatal days (D7 and D30) and identified 87 differentially expressed miRNAs (DE-miRNAs). These six stages were further divided into two longer developmental stages based on heatmap cluster analysis, and the results showed that DE-mRNAs in Stage A were closely related to HF morphogenesis. A coanalysis of Stage A DE-mRNAs and DE-miRNAs revealed that 9 DE-miRNAs and 17 DE-mRNAs presented targeting relationships in Stage A. We found that miR-23b and miR-133 could target and regulate ACVR1B and WNT10A. In dermal fibroblasts, the overexpression of miR-133 significantly reduced the mRNA and protein expression levels of ACVR1B. The overexpression of miR-23b significantly reduced the mRNA and protein expression levels of WNT10A.
Conclusion: This study provides a new reference for understanding the molecular basis of HF development and lays a foundation for further improving sheep HF breeding. miRNAs and target genes related to hair follicular development were found, which provided a theoretical basis for molecular breeding for the culture of fine-wool sheep.
(© 2022. The Author(s).)

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