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Tytuł:
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Phospholipid and antioxidant responses of oleaginous fungus Cunninghamella echinulata against hydrogen peroxide stress.
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Autorzy:
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Li S; Department of Biotechnology, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China; Department of Academic Research, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China.
Feng X; Huazhong Agricultural University, Wuhan, 430070, China.
Zhang X; Department of Biotechnology, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Xie S; Department of Biotechnology, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China. Electronic address: shangxian_.
Ma F; Department of Biotechnology, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China. Electronic address: .
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Źródło:
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Archives of biochemistry and biophysics [Arch Biochem Biophys] 2022 Nov 30; Vol. 731, pp. 109447. Date of Electronic Publication: 2022 Oct 22.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: <2000- > : San Diego, CA : Elsevier
Original Publication: New York, NY : Academic Press
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MeSH Terms:
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Hydrogen Peroxide*/metabolism
Cunninghamella*/metabolism
Phospholipids/metabolism ; Antioxidants/metabolism ; Oxidative Stress/physiology
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Contributed Indexing:
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Keywords: Cunninghamella echinulata; Hydrogen peroxide; Oxidative stress; Phospholipid; Transcriptomics
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Substance Nomenclature:
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BBX060AN9V (Hydrogen Peroxide)
0 (Phospholipids)
0 (Antioxidants)
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SCR Organism:
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Cunninghamella echinulata
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Entry Date(s):
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Date Created: 20221025 Date Completed: 20221109 Latest Revision: 20221211
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Update Code:
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20240105
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DOI:
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10.1016/j.abb.2022.109447
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PMID:
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36283482
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Hydrogen peroxide (H 2 O 2 ) is one of the major oxidative stress intracellularly and extracellularly, which may affect lipid membrane or cell membrane. However, the mechanism remains unclear. The present study investigated phospholipid and antioxidant responses of Cunninghamella echinulata under exogenous H 2 O 2 stress by integrating lipidomics and transcriptomics. H 2 O 2 significantly affected phospholipid profile of C. echinulata exposed to exogenous H 2 O 2 . The phospholipid content was reduced from 6.41% to 2.47% on the first day, and to 1.03% on the 7th day, which was 5-6 times lower than that in the control. Phosphatidyl choline was reduced significantly from 29.71% to 2.73% on the 7th day. The lipid-related metabolic maps of C. echinulata responding to H 2 O 2 were constructed based on transcriptomics, lipidomics and biochemical analysis. Results showed that H 2 O 2 almost mobilized all the signaling pathways in the cell, especially the AMPK and cAMP signaling pathway, which regulated the metabolism of proteins and fatty acids. H 2 O 2 -stress triggered the high expression of heat shock genes. The antioxidant enzymes were activated to supply more NADPH, which contributed to the modulation of intracellular redox balance, and continuously scavenged active substances, thus improving the mycelial resistance to oxidative stress.
Competing Interests: Declaration of competing interest None.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
