Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens.

Tytuł:: Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens.
Autorzy:: Chatelain P; Agroécologie, CNRS, INRAE, Institut Agro, Université de Bourgogne, Université Bourgogne Franche-Comté, Dijon, France.
Blanchard C; Agroécologie, CNRS, INRAE, Institut Agro, Université de Bourgogne, Université Bourgogne Franche-Comté, Dijon, France.
Astier J; Agroécologie, CNRS, INRAE, Institut Agro, Université de Bourgogne, Université Bourgogne Franche-Comté, Dijon, France.
Klinguer A; Agroécologie, CNRS, INRAE, Institut Agro, Université de Bourgogne, Université Bourgogne Franche-Comté, Dijon, France.
Wendehenne D; Agroécologie, CNRS, INRAE, Institut Agro, Université de Bourgogne, Université Bourgogne Franche-Comté, Dijon, France.
Jeandroz S; Agroécologie, CNRS, INRAE, Institut Agro, Université de Bourgogne, Université Bourgogne Franche-Comté, Dijon, France.
Rosnoblet C; Agroécologie, CNRS, INRAE, Institut Agro, Université de Bourgogne, Université Bourgogne Franche-Comté, Dijon, France. .
Źródło:: Scientific reports [Sci Rep] 2022 Nov 08; Vol. 12 (1), pp. 18988. Date of Electronic Publication: 2022 Nov 08.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Gene Expression Regulation, Plant*
Streptophyta*/genetics
Stress, Physiological/genetics ; Genes, Plant ; Salinity ; Real-Time Polymerase Chain Reaction ; Reference Standards ; Gene Expression Profiling
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Grant Information:: ALGAE-NOS ANR-18-CE20-0022-02 Agence Nationale de la Recherche; NOISELESS-RA18041.AEC.IS ISITE-BFC
Entry Date(s):: Date Created: 20221108 Date Completed: 20221110 Latest Revision: 20230105
Update Code:: 20240104
PubMed Central ID:: PMC9643330
DOI:: 10.1038/s41598-022-23783-9
PMID:: 36348043
: Czasopismo naukowe

Pełny tekst

Microalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to its particular adaptation to environmental stresses, represents an interesting photosynthetic eukaryote for studying the transition stages leading to the colonization of terrestrial life. The tolerance to different stresses is manifested by changes in gene expression, which can be monitored by quantifying the amounts of transcripts by RT-qPCR. The identification of optimal reference genes for experiment normalization was therefore necessary. In this study, using four statistical algorithms followed by the RankAggreg package, we determined the best reference gene pairs suitable for normalizing RT-qPCR data in K. nitens in response to three abiotic stresses: high salinity, PEG-induced dehydration and heat shock. Based on these reference genes, we were able to identify marker genes in response to the three abiotic stresses in K. nitens.
(© 2022. The Author(s).)

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