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Tytuł pozycji:

Nile tilapia (Oreochromis niloticus) Nanog co-expression with Pou5f3, transcriptional regulation and biological activity in embyonic development and embryonic cells.

Tytuł:
Nile tilapia (Oreochromis niloticus) Nanog co-expression with Pou5f3, transcriptional regulation and biological activity in embyonic development and embryonic cells.
Autorzy:
Bai X; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China.
Jianeng L; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China.
Zhang Z; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China.
Qu X; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China.
Tao W; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China.
Zhou L; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China.
Wang D; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China. Electronic address: .
Wei J; Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Aquatic Science of Chongqing, School of Life Sciences, Southwest University, 400715 Chongqing, China. Electronic address: .
Źródło:
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2023 Feb-Mar; Vol. 264, pp. 110812. Date of Electronic Publication: 2022 Nov 15.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Oxford : Pergamon
Original Publication: Oxford : Tarrytown, NY : Pergamon ; Elsevier, c1994-
MeSH Terms:
Zebrafish*
Cichlids*/genetics
Cichlids*/metabolism
Animals ; Gene Expression Regulation, Developmental ; Blastula ; Zebrafish Proteins/genetics ; Zebrafish Proteins/metabolism ; Embryonic Development/genetics ; Mammals/metabolism ; Nanog Homeobox Protein/genetics ; Nanog Homeobox Protein/metabolism
Contributed Indexing:
Keywords: CRISPR/Cas9; Nanog; Nile tilapia; Pluripotency; Pou5f3; Yolk syncytial layer; Zebrafish
Substance Nomenclature:
0 (Zebrafish Proteins)
0 (Nanog protein, zebrafish)
0 (Nanog Homeobox Protein)
Entry Date(s):
Date Created: 20221117 Date Completed: 20221220 Latest Revision: 20221222
Update Code:
20240104
DOI:
10.1016/j.cbpb.2022.110812
PMID:
36396033
Czasopismo naukowe
Mammalian Nanog is critical in pluripotency acquisition and maintenance. Nonetheless, a recent report from zebrafish (Danio rerio) suggests that Nanog is not required for embryonic cells which is not like the mammalian homologs, but is necessary for the proper formation of the extra-embryonic yolk syncytial layer (YSL). However, whether its biological function in other fishes is conservative remains to be investigated. Our previous work shows that Nanog from Nile tilapia (Oreochromis niloticus) (termed as Ong thereafter) displays differential spatiotemporal expression patterns from the other teleost fishes including zebrafish. In this study, Ong co-expression with Pou5f3 (another core pluripotent transcription factor), transcriptional regulation and its biological functions during embryonic development and in the survival and proliferation of embryonic cells were investigated. At the blastula stage, both Ong and Pou5f3 were highly expressed in embryonic cells and co-located in the nucleus. After that, the expression of both Ong and Pou5f3 began to decrease at the gastrula stage (24 haf) and then exhibited a differential expression profile at the segmentation stage (28-36 haf). Ong disappeared in embryonic cells and was limited to YSL, whilst Pou5f3 was highly expressed in embryonic cells even some with obvious cytoplasmic distribution. Luciferase assay indicated that Ong was negatively regulated by Pou5f3 and positively regulated by androgen and itself. Ong depletion in fertilized one-cell embryos through CRISPR/Cas9 led to blastula blockage or death, and the survival and proliferation of blastula-derived embryonic cells in vitro failed. Collectively, Ong has similar expression and biological function to Pou5f3 at the blastula stage, which is similar to mammalian homolog but different from zebrafish homolog. These data suggest that the expression patterns and functions of Nanog are not conservative in fishes and vary from species to species. This study enriches our understanding about Nanog and its evolution.
Competing Interests: Declaration of Competing Interest The authors declared that they have no conflicts of interest. The funding agencies had no influence on the experimental design and the conclusions.
(Copyright © 2022. Published by Elsevier Inc.)

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