Evaluation of a Rapid and Simple Method for Assessing Retinal Vessel Structures in Adult Zebrafish.

Tytuł:: Evaluation of a Rapid and Simple Method for Assessing Retinal Vessel Structures in Adult Zebrafish.
Autorzy:: Lee YR; Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea.; Department of Biology, Chungnam National University, Daejeon 34134, Republic of Korea.
Son M; Research Institute for Aerospace Medicine, Inha University, Incheon 22212, Republic of Korea.
Kim YS; Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea.
Kim JS; Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea.; Julia Laboratory, Suwon 16232, Republic of Korea.
Kim CH; Department of Biology, Chungnam National University, Daejeon 34134, Republic of Korea.
Jung SH; Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Republic of Korea.; Department of Genetic Resources, National Marine Biodiversity Institute of Korea (MABIK), Seocheon 33662, Republic of Korea.
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2022 Dec 01; Vol. 23 (23). Date of Electronic Publication: 2022 Dec 01.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:: Retinal Diseases*/metabolism
Zebrafish*
Animals ; Blood-Retinal Barrier ; Endothelial Cells ; Retinal Vessels/metabolism ; Trypsin/metabolism ; Zebrafish Proteins/metabolism
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Grant Information:: K15270 Korea Institute of Oriental Medicine; KSN20213302 Korea Institute of Oriental Medicine; 2022M00400 Ministry of Oceans and Fisheries; 20220027 Ministry of Oceans and Fisheries; 2018R1A6A1A03025523 Ministry of Education
Contributed Indexing:: Keywords: blood–retinal barrier; hyperglycemia; retinal vasculature; tight junction; trypsin digestion; zebrafish
Substance Nomenclature:: EC 3.4.21.4 (Trypsin)
0 (Zebrafish Proteins)
Entry Date(s):: Date Created: 20221211 Date Completed: 20221230 Latest Revision: 20221230
Update Code:: 20240105
PubMed Central ID:: PMC9739661
DOI:: 10.3390/ijms232315069
PMID:: 36499406
: Czasopismo naukowe

Pełny tekst

The evaluation of retinal vascular structures is important for analyzing various ophthalmic diseases. Conventional trypsin digestion was used for separating retinal vasculatures in mouse, rat, and other animal models; however, the trypsin method alone is technically difficult to perform and has not been reported in zebrafish to date. In this study, we introduced a rapid and convenient method that allows the investigation of fine vessel structures at a cellular level in the relatively intact retinal vasculature of adult zebrafish. Using an anti-ZO-1 antibody, tight junction structures in retinal vessels were examined in detail and several different cell types constituting blood vessels in arterial and capillary areas were identified. In addition, using cell type-specific antibodies, we identified smooth muscle cells, blood cells, and endothelial cells in the retinal vasculature. Finally, using the hyperglycemic model, we observed the dilation of retinal vessels, the downregulation of tight junction proteins, and the reduction in smooth muscle cells. Based on these results, we provide a rapid and convenient method for the study of retinal vasculature disease in the zebrafish animal model.

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