Interspecies competition in oral biofilms mediated by Streptococcus gordonii extracellular deoxyribonuclease SsnA.

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Tytuł:: Interspecies competition in oral biofilms mediated by Streptococcus gordonii extracellular deoxyribonuclease SsnA.
Autorzy:: Rostami N; School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.
Shields RC; School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.; Department of Biological Sciences, Arkansas State University, Jonesboro, AR, USA.
Serrage HJ; Bristol Dental School, University of Bristol, Bristol, UK.
Lawler C; Bristol Dental School, University of Bristol, Bristol, UK.
Brittan JL; Bristol Dental School, University of Bristol, Bristol, UK.
Yassin S; School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.; Department of Restorative Sciences, University of Alabama at Birmingham, Birmingham, AL, USA.
Ahmed H; School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.
Treumann A; Protein and Proteome Analysis Facility, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.; KBI Biopharma BV, Leuven, Belgium.
Thompson P; Protein and Proteome Analysis Facility, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.
Waldron KJ; Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle, UK.
Nobbs AH; Bristol Dental School, University of Bristol, Bristol, UK.
Jakubovics NS; School of Dental Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle, UK. .
Źródło:: NPJ biofilms and microbiomes [NPJ Biofilms Microbiomes] 2022 Dec 12; Vol. 8 (1), pp. 96. Date of Electronic Publication: 2022 Dec 12.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [New York, NY ?] : Nature Publishing Group, [2015]-
MeSH Terms:: Streptococcus gordonii*/genetics
Dental Plaque*
Humans ; Streptococcus mutans ; Biofilms ; Saliva
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Grant Information:: R01 DE016690 United States DE NIDCR NIH HHS; BB/S006818/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
Entry Date(s):: Date Created: 20221212 Date Completed: 20221214 Latest Revision: 20230701
Update Code:: 20240105
PubMed Central ID:: PMC9744736
DOI:: 10.1038/s41522-022-00359-z
PMID:: 36509765
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Extracellular DNA (eDNA) is a key component of many microbial biofilms including dental plaque. However, the roles of extracellular deoxyribonuclease (DNase) enzymes within biofilms are poorly understood. Streptococcus gordonii is a pioneer colonizer of dental plaque. Here, we identified and characterised SsnA, a cell wall-associated protein responsible for extracellular DNase activity of S. gordonii. The SsnA-mediated extracellular DNase activity of S. gordonii was suppressed following growth in sugars. SsnA was purified as a recombinant protein and shown to be inactive below pH 6.5. SsnA inhibited biofilm formation by Streptococcus mutans in a pH-dependent manner. Further, SsnA inhibited the growth of oral microcosm biofilms in human saliva. However, inhibition was ameliorated by the addition of sucrose. Together, these data indicate that S. gordonii SsnA plays a key role in interspecies competition within oral biofilms. Acidification of the medium through sugar catabolism could be a strategy for cariogenic species such as S. mutans to prevent SsnA-mediated exclusion from biofilms.
(© 2022. The Author(s).)

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