Elevated Expression and Activation of GPR15 in Immune Cells in Graves' Disease.

Tytuł:: Elevated Expression and Activation of GPR15 in Immune Cells in Graves' Disease.
Autorzy:: Zhao J; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.; Department of Endocrinology, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi 214000, China.
Liu X; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Xu J; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Fang Y; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Du P; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Gao C; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Cai T; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Gu Z; Zhoupu Community Health Service Center of Pudong New Area, Shanghai 201508, China.
Qin Q; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Zhang J; Department of Endocrinology and Rheumatology, Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital, Shanghai 201508, China.
Źródło:: Biomolecules [Biomolecules] 2022 Dec 18; Vol. 12 (12). Date of Electronic Publication: 2022 Dec 18.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, 2011-
MeSH Terms:: Leukocytes, Mononuclear*/metabolism
Graves Disease*/metabolism
Humans ; T-Lymphocytes, Helper-Inducer ; Killer Cells, Natural ; Receptors, Peptide ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism
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Contributed Indexing:: Keywords: GPR15; GPR15L; Graves’ disease; IL21; IL4; Tfh
Substance Nomenclature:: 0 (GPR15 protein, human)
0 (Receptors, Peptide)
0 (Receptors, G-Protein-Coupled)
Entry Date(s):: Date Created: 20221223 Date Completed: 20221226 Latest Revision: 20221227
Update Code:: 20240104
PubMed Central ID:: PMC9776225
DOI:: 10.3390/biom12121899
PMID:: 36551327
: Czasopismo naukowe

Pełny tekst

GPR15 plays an important role in lymphocyte homing and is a key immune molecule to maintain organ immune homeostasis. Yet, no study on the association between GPR15 and Graves' disease (GD) is available. In this study, we systematically investigated the expression of GPR15 in different types of immune cells and different tissues of GD patients. We found that the expressions of GPR15 and GPR15L in peripheral blood of GD patients were increased compared with those in healthy controls. A flow cytometry analysis showed that GPR15 positive cells were mainly CD14+ monocytes and CD56+ natural killer cells (NK cells) of innate immunity, T helper cells and cytotoxic T cells of adaptive immunity. We also found that the expressions of GPR15 and GPR15L in the PBMC of GD patients were positively correlated with the Tfh-specific cytokines IL21 and IL4. In addition, immunohistochemistry showed that the level of GPR15 in thyroid tissue of GD patients was higher than that of the control group. Our results demonstrate for the first time that GPR15 is highly expressed in various immune cells in GD patients, suggesting that GPR15-GPR15L is associated with the activation and infiltration of proinflammatory immune cells in the thyroid tissue of GD patients.

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