DNA Damage in Splenocytes of Mice Exposed to Secondary Radiation Created by 650 MeV Protons Bombarding a Concrete Shielding Barrier.

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Tytuł:: DNA Damage in Splenocytes of Mice Exposed to Secondary Radiation Created by 650 MeV Protons Bombarding a Concrete Shielding Barrier.
Autorzy:: Blokhina TM; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.; N. N. Se-menov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
Ivanov AA; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.; Institute of Biomedical Problems, State Research Center, Russian Academy of Sciences, Moscow, Russia.; United Institute of Nuclear Research, Dubna, Russia.
Vorobyeva NY; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.; N. N. Se-menov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
Yashkina EI; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.; N. N. Se-menov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
Nikitenko OV; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.; Institute of Biomedical Problems, State Research Center, Russian Academy of Sciences, Moscow, Russia.
Bychkova TM; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.; Institute of Biomedical Problems, State Research Center, Russian Academy of Sciences, Moscow, Russia.
Molokanov AG; United Institute of Nuclear Research, Dubna, Russia.
Timoshenko GN; United Institute of Nuclear Research, Dubna, Russia.
Bushmanov AY; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Samoilov AS; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Osipov AN; A. I. Burnasyan Federal Medical Biophysical Center, Federal Medical-Biological Agency of Russia, Moscow, Russia. .; N. N. Se-menov Federal Research Center of Chemical Physics, Russian Academy of Sciences, Moscow, Russia. .
Źródło:: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2022 Dec; Vol. 174 (2), pp. 194-198. Date of Electronic Publication: 2023 Jan 05.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: New York : Springer
Original Publication: New York, Consultants Bureau.
MeSH Terms:: Protons*
Spleen*
Mice ; Animals ; DNA Damage ; Radiation, Ionizing ; DNA
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Contributed Indexing:: Keywords: DNA double-strand breaks; apoptosis; mouse splenocytes; protons; secondary ionizing radiation
Substance Nomenclature:: 0 (Protons)
9007-49-2 (DNA)
Entry Date(s):: Date Created: 20230104 Date Completed: 20230112 Latest Revision: 20230112
Update Code:: 20240105
DOI:: 10.1007/s10517-023-05672-x
PMID:: 36600036
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The proportion of splenocytes with a high level of DNA double-strand breaks was determined in mice exposed to primary and secondary radiation created by bombarding of a concrete barrier (thickness 20, 40, and 80 cm) by 650 MeV protons. The proportion of splenocytes with a high level of DNA double-strand breaks was assessed by flow cytometric analysis of γH2AX + and TUNEL + cells. It is shown that concrete barrier can significantly reduce primary proton radiation; the severity of negative biological effects in mice irradiated in the center of the proton beam decreased with increasing the thickness of this barrier. However, the spectrum of secondary radiation changes significantly with increasing the barrier thickness from 20 to 80 cm and the distance from central axis of the beam from 0 to 20 cm, and the proportion of the neutron component increases, which also causes negative biological effects manifesting in a significant (p<0.05) increase in the percentage of splenocytes with a high level of DNA damage in mice irradiated at a distance of 20 cm from the center of the proton beam and receiving relatively low doses (0.10-0.17 Gy).
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