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Tytuł:
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Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
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Autorzy:
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Rübsam H; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Krönauer C; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Abel NB; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Ji H; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.; National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Lironi D; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Hansen SB; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Nadzieja M; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Kolte MV; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Abel D; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
de Jong N; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Madsen LH; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Liu H; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Stougaard J; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Radutoiu S; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Andersen KR; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
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Źródło:
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Science (New York, N.Y.) [Science] 2023 Jan 20; Vol. 379 (6629), pp. 272-277. Date of Electronic Publication: 2023 Jan 19.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-
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MeSH Terms:
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Root Nodules, Plant*/metabolism
Signal Transduction*
Symbiosis*/physiology
Single-Domain Antibodies*
Lotus*
Lipopolysaccharides*/metabolism
Cell Membrane/metabolism ; Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Medicago truncatula
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Substance Nomenclature:
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0 (Plant Proteins)
0 (Single-Domain Antibodies)
0 (Lipopolysaccharides)
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Entry Date(s):
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Date Created: 20230119 Date Completed: 20230221 Latest Revision: 20230221
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Update Code:
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20240104
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DOI:
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10.1126/science.ade9204
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PMID:
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36656954
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Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
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