Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations.

Tytuł:: Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations.
Autorzy:: Esimbekova EN; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Laboratory of Photobiology, Institute of Biophysics of Siberian Branch of Russian Academy of Science, 660036 Krasnoyarsk, Russia.
Kalyabina VP; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Laboratory of Photobiology, Institute of Biophysics of Siberian Branch of Russian Academy of Science, 660036 Krasnoyarsk, Russia.
Kopylova KV; Laboratory of Cell Molecular Physiology and Pathology, Scientific Research Institute of Medical Problems of the North of the Siberian Branch of the Russian Academy of Sciences, 660022 Krasnoyarsk, Russia.
Lonshakova-Mukina VI; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Laboratory of Photobiology, Institute of Biophysics of Siberian Branch of Russian Academy of Science, 660036 Krasnoyarsk, Russia.
Antashkevich AA; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.
Torgashina IG; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.
Lukyanenko KA; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Laboratory for Digital Controlled Drugs and Theranostics, Federal Research Center 'Krasnoyarsk Research Center' of Siberian Branch of Russian Academy of Science, 660036 Krasnoyarsk, Russia.; Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University after Prof. V.F. Voino-Yasenetsky, 660022 Krasnoyarsk, Russia.
Nemtseva EV; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Laboratory of Photobiology, Institute of Biophysics of Siberian Branch of Russian Academy of Science, 660036 Krasnoyarsk, Russia.
Kratasyuk VA; Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia.; Laboratory of Photobiology, Institute of Biophysics of Siberian Branch of Russian Academy of Science, 660036 Krasnoyarsk, Russia.
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2023 Jan 23; Vol. 24 (3). Date of Electronic Publication: 2023 Jan 23.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:: Pesticides*/toxicity
Pesticides*/analysis
Ecosystem ; Photobacterium ; Biological Assay/methods
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Grant Information:: Strategic Project "Gastronomic RnD Park" Siberian Federal University; 0287-2021-0020 Ministry of Science and Higher Education of the Russian Federation
Contributed Indexing:: Keywords: bioluminescent assay; conjugated enzyme reactions; enzyme inhibition-based assay; formulants; luminous bacteria; pesticides
Substance Nomenclature:: 0 (Pesticides)
SCR Organism:: Photobacterium phosphoreum
Entry Date(s):: Date Created: 20230211 Date Completed: 20230214 Latest Revision: 20230214
Update Code:: 20240105
PubMed Central ID:: PMC9916951
DOI:: 10.3390/ijms24032268
PMID:: 36768591
: Czasopismo naukowe

Pełny tekst

Pesticides can affect the health of individual organisms and the function of the entire ecosystem. Therefore, thorough assessment of the risks associated with the use of pesticides is a high-priority task. An enzyme inhibition-based assay is used in this study as a convenient and quick tool to study the effects of pesticides at the molecular level. The contribution of formulants to toxicological properties of the pesticide formulations has been studied by analyzing effects of 7 active ingredients of pesticides (AIas) and 10 commercial formulations based on them (AIfs) on the function of a wide range of enzyme assay systems differing in complexity (single-, coupled, and three-enzyme assay systems). Results have been compared with the effects of AIas and AIfs on bioluminescence of the luminous bacterium Photobacterium phosphoreum . Mostly, AIfs produce a considerably stronger inhibitory effect on the activity of enzyme assay systems and bioluminescence of the luminous bacterium than AIas, which confirms the contribution of formulants to toxicological properties of the pesticide formulation. Results of the current study demonstrate that "inert" ingredients are not ecotoxicologically safe and can considerably augment the inhibitory effect of pesticide formulations; therefore, their use should be controlled more strictly. Circular dichroism and fluorescence spectra of the enzymes used for assays do not show any changes in the protein structure in the presence of commercial pesticide formulations during the assay procedure. This finding suggests that pesticides produce the inhibitory effect on enzymes through other mechanisms.

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