Canine distemper virus N protein induces autophagy to facilitate viral replication.

Tytuł:: Canine distemper virus N protein induces autophagy to facilitate viral replication.
Autorzy:: Chen F; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xu Jiaping, Lanzhou, 730046, Gansu, China.
Guo Z; College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, 16 Yihuan Rd., Chengdu, 610041, Sichuan, China.
Zhang R; College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, 16 Yihuan Rd., Chengdu, 610041, Sichuan, China.
Zhang Z; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xu Jiaping, Lanzhou, 730046, Gansu, China.
Hu B; Key Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, 4899 Juye St., Changchun, 130112, Jilin, China.
Bai L; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xu Jiaping, Lanzhou, 730046, Gansu, China.
Zhao S; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xu Jiaping, Lanzhou, 730046, Gansu, China.
Wu Y; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 1 Xu Jiaping, Lanzhou, 730046, Gansu, China.
Zhang Z; College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, 16 Yihuan Rd., Chengdu, 610041, Sichuan, China. .
Li Y; College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, 16 Yihuan Rd., Chengdu, 610041, Sichuan, China. .
Źródło:: BMC veterinary research [BMC Vet Res] 2023 Mar 15; Vol. 19 (1), pp. 60. Date of Electronic Publication: 2023 Mar 15.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: London : BioMed Central, 2005-
MeSH Terms:: Distemper*
Distemper Virus, Canine*/physiology
Dog Diseases*/virology
Virus Replication*
Nucleocapsid Proteins*/metabolism
Animals ; Dogs ; Autophagy ; Chlorocebus aethiops ; Vero Cells
References:: Antiviral Res. 2015 Oct;122:1-11. (PMID: 26210812)
Virology. 2010 Sep 30;405(2):383-9. (PMID: 20605567)
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14046-51. (PMID: 19666601)
Infect Genet Evol. 2016 Jul;41:262-269. (PMID: 27101783)
FEBS J. 2016 Feb;283(4):576-94. (PMID: 26684000)
Cell Host Microbe. 2009 Oct 22;6(4):354-66. (PMID: 19837375)
Cell. 2005 May 20;121(4):567-577. (PMID: 15907470)
DNA Cell Biol. 2018 Apr;37(4):287-290. (PMID: 29350547)
Cell Host Microbe. 2014 May 14;15(5):564-77. (PMID: 24832451)
Annu Rev Genet. 2009;43:67-93. (PMID: 19653858)
PLoS Biol. 2005 May;3(5):e156. (PMID: 15884975)
Viruses. 2016 Oct 11;8(10):. (PMID: 27727184)
Biochim Biophys Acta. 2000 Apr 17;1496(2-3):207-20. (PMID: 10771089)
J Virol. 2014 Apr;88(8):4423-33. (PMID: 24501402)
Arch Virol. 2019 Apr;164(4):1233-1244. (PMID: 30663023)
PLoS Pathog. 2013;9(9):e1003599. (PMID: 24086130)
Autophagy. 2013 Feb 1;9(2):124-37. (PMID: 23295650)
J Cell Biol. 2005 May 9;169(3):425-34. (PMID: 15866887)
Curr Mol Med. 2013 Feb;13(2):305-16. (PMID: 23228131)
J Alzheimers Dis. 2012;30(4):815-31. (PMID: 22475795)
Autophagy. 2022 Apr;18(4):816-828. (PMID: 34338134)
Mol Cell. 2010 Oct 22;40(2):280-93. (PMID: 20965422)
Front Immunol. 2018 Sep 12;9:2096. (PMID: 30258449)
PLoS One. 2012;7(10):e48264. (PMID: 23110223)
Virus Res. 2020 Dec;290:198164. (PMID: 32949657)
J Virol. 2018 Mar 28;92(8):. (PMID: 29386287)
BMC Vet Res. 2015 Jul 25;11:164. (PMID: 26205391)
Cell Host Microbe. 2009 Oct 22;6(4):367-80. (PMID: 19837376)
Viruses. 2016 Jul 28;8(8):. (PMID: 27483301)
Int J Mol Med. 2017 Jul;40(1):182-192. (PMID: 28560385)
Biotech Histochem. 2015;90(8):601-7. (PMID: 26179070)
Nat Rev Microbiol. 2004 Apr;2(4):301-14. (PMID: 15031729)
Autophagy. 2018;14(2):336-346. (PMID: 29166823)
Transbound Emerg Dis. 2020 Jul;67 Suppl 2:178-184. (PMID: 32080984)
Immunity. 2009 Apr 17;30(4):588-98. (PMID: 19362021)
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11345-50. (PMID: 9326612)
J Genet Genomics. 2011 Nov 20;38(11):533-7. (PMID: 22133684)
Autophagy. 2016;12(1):1-222. (PMID: 26799652)
Nature. 2006 Jun 15;441(7095):885-9. (PMID: 16625204)
Autophagy. 2020 May;16(5):842-861. (PMID: 31318632)
J Virol. 2012 Aug;86(16):8527-35. (PMID: 22647692)
Cell. 2010 Feb 5;140(3):313-26. (PMID: 20144757)
Rev Med Virol. 2009 Nov;19(6):359-78. (PMID: 19750559)
Cell. 2017 Mar 9;168(6):960-976. (PMID: 28283069)
J Biol Chem. 2013 Feb 1;288(5):3571-84. (PMID: 23233667)
Autophagy. 2012 Oct;8(10):1434-47. (PMID: 22739997)
Autophagy. 2016 Oct 2;12(10):1704-1720. (PMID: 27463027)
Vet Clin North Am Small Anim Pract. 2011 Nov;41(6):1069-86. (PMID: 22041204)
J Viral Hepat. 2011 Dec;18(12):821-30. (PMID: 22093031)
Virol J. 2019 Mar 7;16(1):30. (PMID: 30845967)
Viruses. 2014 Jul 02;6(7):2571-601. (PMID: 24992230)
Nat Rev Mol Cell Biol. 2007 Sep;8(9):741-52. (PMID: 17717517)
Virology. 2010 Sep 15;405(1):1-7. (PMID: 20580051)
Autophagy. 2007 May-Jun;3(3):181-206. (PMID: 17224625)
J Virol. 2014 Jan;88(1):525-37. (PMID: 24173218)
J Gen Virol. 1990 Jul;71 ( Pt 7):1585-90. (PMID: 2374009)
Hepatology. 2008 Oct;48(4):1054-61. (PMID: 18688877)
mBio. 2017 Jun 13;8(3):. (PMID: 28611246)
J Virol. 2008 Sep;82(18):9143-53. (PMID: 18596087)
J Mol Med (Berl). 2007 Aug;85(8):811-23. (PMID: 17340132)
Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5435-40. (PMID: 22431644)
Cell Host Microbe. 2010 Feb 18;7(2):115-27. (PMID: 20159618)
Curr Top Microbiol Immunol. 2009;335:1-32. (PMID: 19802558)
Viruses. 2019 Jun 26;11(7):. (PMID: 31247987)
Nat Immunol. 2009 May;10(5):461-70. (PMID: 19381141)
Emerg Infect Dis. 2010 Dec;16(12):2007-9. (PMID: 21122248)
Nat Rev Mol Cell Biol. 2007 Nov;8(11):931-7. (PMID: 17712358)
Grant Information:: 2021115; 125900/16011211013 Southwest Minzu University Research Startup Funds; 2021115; 125900/16011211013 Southwest Minzu University Research Startup Funds; 2022NSFSC0073 Natural Science Foundation of Sichuan Province; 20YF3WA008 Gansu Province Science and Technology Planning Project
Contributed Indexing:: Keywords: Autophagy; Canine distemper virus; Nucleocapsid protein; Replication
Substance Nomenclature:: 0 (Nucleocapsid Proteins)
Entry Date(s):: Date Created: 20230316 Date Completed: 20230322 Latest Revision: 20230322
Update Code:: 20240105
PubMed Central ID:: PMC10015816
DOI:: 10.1186/s12917-023-03575-7
PMID:: 36922800
: Czasopismo naukowe

Pełny tekst

Background: Canine distemper virus (CDV) is one of the most contagious and lethal viruses known to the Canidae, with a very broad and expanding host range. Autophagy serves as a fundamental stabilizing response against pathogens, but some viruses have been able to evade or exploit it for their replication. However, the effect of autophagy mechanisms on CDV infection is still unclear.
Results: In the present study, autophagy was induced in CDV-infected Vero cells as demonstrated by elevated LC3-II levels and aggregation of green fluorescent protein (GFP)-LC3 spots. Furthermore, CDV promoted the complete autophagic process, which could be determined by the degradation of p62, co-localization of LC3 with lysosomes, GFP degradation, and accumulation of LC3-II and p62 due to the lysosomal protease inhibitor E64d. In addition, the use of Rapamycin to promote autophagy promoted CDV replication, and the inhibition of autophagy by Wortmannin, Chloroquine and siRNA-ATG5 inhibited CDV replication, revealing that CDV-induced autophagy facilitated virus replication. We also found that UV-inactivated CDV still induced autophagy, and that nucleocapsid (N) protein was able to induce complete autophagy in an mTOR-dependent manner.
Conclusions: This study for the first time revealed that CDV N protein induced complete autophagy to facilitate viral replication.
(© 2023. The Author(s).)

Zaloguj się, aby uzyskać dostęp do pełnego tekstu.

Informacja