Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Comparison of methods for detecting protein extracted from excess activated sludge.

Tytuł:
Comparison of methods for detecting protein extracted from excess activated sludge.
Autorzy:
Yan Y; School of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, China.
Zhang M; School of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, China.
Gao J; School of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, China. .
Qin L; School of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, China.
Fu X; School of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, China.
Wan J; School of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, China.; Henan International Joint Laboratory of Environment and Resources, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
Źródło:
Environmental science and pollution research international [Environ Sci Pollut Res Int] 2023 May; Vol. 30 (21), pp. 60967-60975. Date of Electronic Publication: 2023 Apr 12.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: <2013->: Berlin : Springer
Original Publication: Landsberg, Germany : Ecomed
MeSH Terms:
Sewage*/chemistry
Proteins*/chemistry
Reproducibility of Results ; Hydrolysis ; Protein Hydrolysates
References:
Chen M, Zhou H, Zhu X, Tan Z, Li X (2015) Optimization of determination of protein in activated sludge by Bradford method. Environ Sci Technol 38:1–5.
Collivignarelli MC, Castagnola F, Sordi M, Bertanza G (2017) Sewage sludge treatment in a thermophilic membrane reactor (TMR): factors affecting foam formation. Environ Sci Pollut Res 24:2316–2325. (PMID: 10.1007/s11356-016-7983-4)
Dobrowolska-Iwanek J (2015) Simple method for determination of short-chain organic acid in mead[J]. Food Anal Methods 8(9):2356–2359. (PMID: 10.1007/s12161-015-0127-5)
Gao J, Wang Y, Yan Y, Li Z, Chen M (2020) Protein extraction from excess sludge by alkali-thermal hydrolysis. Environ Sci Pollut Res 27:8628–8637. (PMID: 10.1007/s11356-019-07188-2)
Gao J, Weng W, Yan Y, Wang Y, Wang Q (2020) Comparison of protein extraction methods from excess activated sludge. Chemosphere 249:126107. (PMID: 10.1016/j.chemosphere.2020.126107)
García M, Urrea JL, Collado S (2017) Protein recovery from solubilized sludge by hydrothermal treatments. Waste Manag 67:278–287. (PMID: 10.1016/j.wasman.2017.05.051)
Hall NG, Schönfeldt HC (2013) Total nitrogen vs. amino-acid profile as indicator of protein content of beef. Food Chem 140:608–612. (PMID: 10.1016/j.foodchem.2012.08.046)
Hao J (2015) The research of optimizing the hydrolysis the excess sludge by compound enzymes and the rule of transportation and transformation of heavy metal(D). Donghua University, Shanghai.
He P, Zhang Y (2019) A review of methods for determination of polysaccharide. Modern Food 02:27–31.
Hwang J, Zhang L, Seo S, Lee Y, Jahng D (2008) Protein recovery from excess sludge for its use as animal feed. Bioresource Technol 99:8949–8954. (PMID: 10.1016/j.biortech.2008.05.001)
Jiang X, Lu Y, Yang H, Yu A, Wang X (2013) Determination of protein extract in potato by Lowry method. Pharm J Chin People’s Liberation Army 29:348–350.
Kumar R, Shukla AK, Bagga E, Kumari S, Bajpai RP, Bharadwaj LM (2005) 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method. Anal Biochem 336:132–134. (PMID: 10.1016/j.ab.2004.09.038)
Le C, Kunacheva C, Stuckey DC (2016) “Protein” measurement in biological wastewater treatment systems: a critical evaluation. Environ Sci Technol 50:3074–3081. (PMID: 10.1021/acs.est.5b05261)
Li P, Li D, Su R, Yan G (2011) Study on hydrolysis of by two excess sludge protein treatment methods. Chin J Environ Eng 5:2859–2863.
Li P, Yan G, Li D, Su R, Chen Z (2012) Determination of protein content in sludge with rapid selenium powder catalysis method. Chem World 53:151–153.
Li Z (2017) Chemical hydrolysis for protein extraction from thickened sludge and its dewatering profile. Zhengzhou University.
Li B, Zhang H (2000) Discussion on the experimental scheme of standard spiked recovery. Chin J Health Lab Technol 05:618–619.
Liu Y, Kong S, Li Y, Zeng H (2009) Novel technology for sewage sludge utilization: preparation of amino acids chelated trace elements (AACTE) fertilizer. J Hazard Mater 171:1159–1167. (PMID: 10.1016/j.jhazmat.2009.06.123)
Lu J, Li D (2015) Extraction of proteins from excess activated sludge by ultrasound combined with composite enzymatic hydrolysis. Environ Sci Technol 38:53–58.
Lu Y, Ma Y, Feng Z, Yu F, Liu C, Yi Y (2010) Bicinchoninic acid method for determination of protein content in milk. Food Sci 31:151–154.
Lucarini AC, Kilikian BV (1999) Comparative study of Lowry and Bradford methods: interfering substances. Biotechnol Tech 13:149–154. (PMID: 10.1023/A:1008995609027)
Marcó A, Rubio R, Compañó R, Casals I (2002) Comparison of the Kjeldahl method and a combustion method for total nitrogen determination in animal feed. Talanta 57:1019–1026. (PMID: 10.1016/S0039-9140(02)00136-4)
Mei X, Tang J, Zhang Y (2018) Transformation mechanism of the sludge stabilization process and the products value in municipal wastewater treatment plant. Water Wastewater Eng 54:11–19.
Miao C (2017) Study on enzymatic hydrolysis of sludge enhanced by ultrasound(D). Zhengzhou University, Zhengzhou.
Navarro JA, Granadillo VA, Parra OE et al (1989) Determination of lead in whole blood by graphite furnace atomic absorption spectrometry with matrix modification. J Anal At Spectrom 4(5):401–406. (PMID: 10.1039/ja9890400401)
Peng G, Ye F, Li Y (2012) Investigation of extracellular polymer substances (EPS) and physicochemical properties of activated sludge from different municipal and industrial wastewater treatment plants. Environ Technol 33:857–863. (PMID: 10.1080/09593330.2011.601763)
Ras M, Girbal-Neuhauser E, Paul E, Spérandio M, Lefebvre D (2008) Protein extraction from activated sludge: an analytical approach. Water Res 42:1867–1878. (PMID: 10.1016/j.watres.2007.11.011)
Rosen H (1957) A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys 67:10–15. (PMID: 10.1016/0003-9861(57)90241-2)
Shrivastaw KP, Jhamb SS, Kumar A (1995) Quantitation of the protein content of diphtheria and tetanus toxoids by the Biuret method during production of combined vaccines. Biol 23:61–63. (PMID: 10.1016/1045-1056(95)90013-6)
Silvério SC, Moreira S, Milagres AMF, Macedo EA, Teixeira JA, Mussatto SI (2012) Interference of some aqueous two-phase system phase-forming components in protein determination by the Bradford method. Anal Biochem 421:719–724. (PMID: 10.1016/j.ab.2011.12.020)
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85. (PMID: 10.1016/0003-2697(85)90442-7)
Tyagi VK, Lo SL, Rajpal A (2014) Chemically coupled microwave and ultrasonic pre-hydrolysis of pulp and paper mill waste-activated sludge: effect on sludge solubilisation and anaerobic digestion. Environ Sci Pollut Res 21:6205–6217. (PMID: 10.1007/s11356-013-2426-y)
Vakondios N, Koukouraki EE, Diamadopoulos E (2014) Effluent organic matter (EfOM) characterization by simultaneous measurement of proteins and humic matter. Water Res 63:62–70. (PMID: 10.1016/j.watres.2014.06.011)
Xu Y, Fan H, Zhao D, Su W, Feng Y, Xiao F, Zhang J, Wang D (2022) Optimization of extraction method for water-soluble protein determination by Coomassie bright blue method. Soybean Sci 41:196–202.
Xue Y, Zuo J, Cui J, Sun X (2014) Sludge hydrolysis treatment and microbial protein resource utilization. China Water Wastewater 30:102–104.
Yan P, Yang Q, Wang H, Xie J, Liu D, Niu B (2006) Comparison of Lowry’s modified assay with Bradford’s dye-binding assay for determining proteins in earthworm extract. J Shanxi Med Univ 037:9–11.
Yan Y, Qin L, Gao J, Nan R, Gao J (2020) Protein extraction and sludge dewatering performance of ultrasound-assisted enzymatic hydrolysis of excess sludge. Environ Sci Pollut Res 27:18317–18328. (PMID: 10.1007/s11356-020-08208-2)
Zhang W, Su R, Li D (2012) Extraction of proteins from excess activated sludge by enzymatic hydrolysis. Environ Sci Technol 35:7–10.
Zhou Z, Li C, Li T, Lin L, Peng Y, Wu F, Zou W (2013) Optimization and verification of BCA method for determination of protein content in human coagulation factor VIII. Chin J Biol 26:1488–1492.
Zhu Y, Zheng L, Zhang Y, Zhan H, Tian S (2011) Comparison of methods for determination of protein content in feed. Cereal & Feed Industry 01:59–61.
Grant Information:
2021YFD1700900 the National Key Research and Development Program of China; Program No.22A610006 the Key Scientific Research Project of Higher Education in Henan Province
Contributed Indexing:
Keywords: Bicinchoninic acid; Biological hydrolysis; Bradford; Chemical hydrolysis; Lowry; Protein detection
Substance Nomenclature:
0 (Sewage)
0 (Proteins)
0 (Protein Hydrolysates)
Entry Date(s):
Date Created: 20230412 Date Completed: 20230510 Latest Revision: 20230510
Update Code:
20240105
DOI:
10.1007/s11356-023-26455-x
PMID:
37042919
Czasopismo naukowe
The protein contents of hydrolyzed sludge supernatant are commonly determined with the Kjeldahl method, but this method suffers from complicated operations, long process times, and large quantities of chemicals consumed. In this paper, the Lowry, bicinchoninic acid (BCA), and Bradford methods were used to test the precision and spiked recovery of proteins from sludge supernatants hydrolyzed by alkaline-thermal hydrolysis (ATH), enzymatic hydrolysis (EH), and ultrasound-assisted enzymatic hydrolysis (UEH), and the results were compared with those obtained with the Kjeldahl method. For all the hydrolytic processes, the sludge protein values determined with the three tested methods were within 0.05 of each other, which met the experimental requirement for accuracy. Both the Lowry and BCA methods had recovery rates of 95-105%, while the Bradford method showed large deviations and was not highly reliable. The three protein determination methods showed significant differences with the Kjeldahl method (P<0.05). However, the relative deviation between the Kjeldahl and BCA methods was the smallest (3-5%), followed by those between the Kjeldahl and the Lowry (11-21%) and Bradford methods (21-90%), and the causes of the deviations were analyzed based on the protein hydrolysate components and the mechanisms for the different detection methods. On the basis of these results, the BCA method was chosen as the most appropriate quantification method for use with sludge protein extraction, and it was used to analyze the protein contents extracted from residual sludge samples obtained from two sewage treatment plants. The reliability of the method was verified, and this lays a foundation for the extraction and reclamation of sludge proteins.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies