Complex Formation of an RNA Aptamer with a Part of HIV-1 Tat through Induction of Base Triples in Living Human Cells Proven by In-Cell NMR.

Tytuł:: Complex Formation of an RNA Aptamer with a Part of HIV-1 Tat through Induction of Base Triples in Living Human Cells Proven by In-Cell NMR.
Autorzy:: Eladl O; Structural Energy Bioscience Research Section, Institute of Advanced Energy, Kyoto University, Kyoto 611-0011, Japan.; Graduate School of Energy Science, Kyoto University, Kyoto 606-8501, Japan.; Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
Yamaoki Y; Structural Energy Bioscience Research Section, Institute of Advanced Energy, Kyoto University, Kyoto 611-0011, Japan.; Graduate School of Energy Science, Kyoto University, Kyoto 606-8501, Japan.; Integrated Research Center for Carbon Negative Science, Institute of Advanced Energy, Kyoto University, Uji 611-0011, Japan.
Kondo K; Structural Energy Bioscience Research Section, Institute of Advanced Energy, Kyoto University, Kyoto 611-0011, Japan.; Integrated Research Center for Carbon Negative Science, Institute of Advanced Energy, Kyoto University, Uji 611-0011, Japan.; Biomass Product Tree Industry-Academia Collaborative Research Laboratory, Kyoto University, Kyoto 611-0011, Japan.
Nagata T; Structural Energy Bioscience Research Section, Institute of Advanced Energy, Kyoto University, Kyoto 611-0011, Japan.; Graduate School of Energy Science, Kyoto University, Kyoto 606-8501, Japan.; Integrated Research Center for Carbon Negative Science, Institute of Advanced Energy, Kyoto University, Uji 611-0011, Japan.
Katahira M; Structural Energy Bioscience Research Section, Institute of Advanced Energy, Kyoto University, Kyoto 611-0011, Japan.; Graduate School of Energy Science, Kyoto University, Kyoto 606-8501, Japan.; Integrated Research Center for Carbon Negative Science, Institute of Advanced Energy, Kyoto University, Uji 611-0011, Japan.; Biomass Product Tree Industry-Academia Collaborative Research Laboratory, Kyoto University, Kyoto 611-0011, Japan.
Źródło:: International journal of molecular sciences [Int J Mol Sci] 2023 May 22; Vol. 24 (10). Date of Electronic Publication: 2023 May 22.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Basel, Switzerland : MDPI, [2000-
MeSH Terms:: Aptamers, Nucleotide*/chemistry
HIV-1*/metabolism
Humans ; tat Gene Products, Human Immunodeficiency Virus/metabolism ; Nucleic Acid Conformation ; Magnetic Resonance Spectroscopy ; RNA, Viral/genetics
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Grant Information:: 20H03192, 20K21477, 21H05519, and 22H05596 to M. K., 17H05878 and 20K06524 to T. N., and 19K16054 and 22K05314 to Y. Y.) Japan Society for the Promotion of Science; (20fk0410027 and 23fk0410048 to M. K., and 22ak0101097 to T. N.) Japan Agency for Medical Research and Development; NMRCR-22-05 to T. N. The Collaborative Research Program of the Institute for Protein Research, Osaka University; to Y.Y The Collaboration Program of the Laboratory for Complex Energy Processes, Institute of Ad-vanced Energy, Kyoto University; 235181 to O.E Ministry of Education, Culture, Sports, Science and Technology
Contributed Indexing:: Keywords: RNA aptamer; RNA–protein interaction; Tat; base triple; functional RNA; in-cell NMR
Substance Nomenclature:: 0 (tat Gene Products, Human Immunodeficiency Virus)
0 (Aptamers, Nucleotide)
0 (RNA, Viral)
Entry Date(s):: Date Created: 20230527 Date Completed: 20230529 Latest Revision: 20230529
Update Code:: 20240105
PubMed Central ID:: PMC10218998
DOI:: 10.3390/ijms24109069
PMID:: 37240414
: Czasopismo naukowe

Pełny tekst

An RNA aptamer that strongly binds to a target molecule has the potential to be a nucleic acid drug inside living human cells. To investigate and improve this potential, it is critical to elucidate the structure and interaction of RNA aptamers inside living cells. We examined an RNA aptamer for HIV-1 Tat (TA), which had been found to trap Tat and repress its function in living human cells. We first used in vitro NMR to examine the interaction between TA and a part of Tat containing the binding site for trans-activation response element (TAR). It was revealed that two U-A∗U base triples are formed in TA upon binding of Tat. This was assumed to be critical for strong binding. Then, TA in complex with a part of Tat was incorporated into living human cells. The presence of two U-A∗U base triples was also revealed for the complex in living human cells by in-cell NMR. Thus, the activity of TA in living human cells was rationally elucidated by in-cell NMR.

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