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Tytuł:
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[Fractional protein makeup of Candida utilis yeast protoplasts in the process of their growth and development].
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Autorzy:
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Gurina LV
Petrikevich SB
Shul'ga AV
Rylkin SS
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Transliterated Title:
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Izuchenie fraktsionnogo sostava belka protoplastov drozhzheĭ Candida utilis v protsesse ikh rosta i razvitiia.
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Źródło:
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Mikrobiologiia [Mikrobiologiia] 1979 Mar-Apr; Vol. 48 (2), pp. 245-8.
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Typ publikacji:
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Journal Article
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Język:
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Russian
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Imprint Name(s):
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Publication: Moskva : Izdatelstvo Nauka
Original Publication: Moskva.
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MeSH Terms:
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Candida/*analysis
Fungal Proteins/*analysis
Protoplasts/*analysis
Amino Acids/analysis ; Candida/growth & development
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Substance Nomenclature:
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0 (Amino Acids)
0 (Fungal Proteins)
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Entry Date(s):
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Date Created: 19790301 Date Completed: 19790725 Latest Revision: 20201209
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Update Code:
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20240104
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PMID:
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571510
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Candida utilis IBFMY-405 was grown in a synthetic medium with glucose. Cells taken at the logarithmic phase of growth were studied. The cells were treated with the enzyme from Helix pomatia to prepare protoplasts which were separated by differential centrifugation into groups according to their size. Three protein fractions were isolated from each group and the amino acid composition of the proteins was determined. Proteins of the first fraction (cytoplasmic) prevailed in all of the protoplast groups while the content of proteins of the second fraction (intermediate or myosin-like) was the lowest. As the size of protoplasts increased, difference in the quantitative content of proteins from the first and second fractions became less pronounced. The content of proteins of the third fraction was 3.6 and 2.4 times higher in the protoplasts of the medium size than in the largest protoplasts. The amino acid composition of each protein fraction differed quantitatively and qualitatively in all of the protoplast groups.