A structural basis of the interactions between leucine-rich repeats and protein ligands.

Szczegóły
Abstrakt

Tytuł:: A structural basis of the interactions between leucine-rich repeats and protein ligands.
Autorzy:: Kobe B; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235-9050.
Deisenhofer J
Źródło:: Nature [Nature] 1995 Mar 09; Vol. 374 (6518), pp. 183-6.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Basingstoke : Nature Publishing Group
Original Publication: London, Macmillan Journals ltd.
MeSH Terms:: Leucine/*chemistry
Proteins/*chemistry
Ribonuclease, Pancreatic/*chemistry
Animals ; Cattle ; Computer Graphics ; Crystallography, X-Ray ; Intracellular Signaling Peptides and Proteins ; Models, Molecular ; Protein Binding ; Protein Conformation ; Protein Structure, Secondary ; Ribonuclease, Pancreatic/antagonists & inhibitors
Substance Nomenclature:: 0 (Intracellular Signaling Peptides and Proteins)
0 (Proteins)
39369-21-6 (ribonuclease inhibitor, porcine)
EC 3.1.27.5 (Ribonuclease, Pancreatic)
GMW67QNF9C (Leucine)
Entry Date(s):: Date Created: 19950309 Date Completed: 19950406 Latest Revision: 20191210
Update Code:: 20240104
DOI:: 10.1038/374183a0
PMID:: 7877692
: Czasopismo naukowe

Full Text Finder

The leucine-rich repeat is a recently characterized structural motif used in molecular recognition processes as diverse as signal transduction, cell adhesion, cell development, DNA repair and RNA processing. We present here the crystal structure at 2.5 A resolution of the complex between ribonuclease A and ribonuclease inhibitor, a protein built entirely of leucine-rich repeats. The unusual non-globular structure of ribonuclease inhibitor, its solvent-exposed parallel beta-sheet and the conformational flexibility of the structure are used in the interaction; they appear to be the principal reasons for the effectiveness of leucine-rich repeats as protein-binding motifs. The structure can serve as a model for the interactions of other proteins containing leucine-rich repeats with their ligands.

Informacja