Regional assignment of seven loci to 12p13.2-pter by PCR analysis of somatic cell hybrids containing the der(12) or the der(X) chromosome from a mesothelioma showing t(X;12)(q22;p13).

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Tytuł:: Regional assignment of seven loci to 12p13.2-pter by PCR analysis of somatic cell hybrids containing the der(12) or the der(X) chromosome from a mesothelioma showing t(X;12)(q22;p13).
Autorzy:: Aerssens J; Arthritis and Metabolic Bone Disease Research Unit, Center for Human Genetics-K.U., Leuven, Belgium.
Chaffanet M
Baens M
Matthijs G
Van den Berghe H
Cassiman JJ
Marynen P
Źródło:: Genomics [Genomics] 1994 Mar 01; Vol. 20 (1), pp. 119-21.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: San Diego : Academic Press, [c1987-
MeSH Terms:: Chromosomes, Human, Pair 12*
Translocation, Genetic*
X Chromosome*
Mesothelioma/*genetics
Animals ; Chromosome Mapping ; Genetic Markers ; Humans ; Hybrid Cells/ultrastructure ; In Situ Hybridization, Fluorescence ; Mice ; Polymerase Chain Reaction ; Sequence Tagged Sites
Substance Nomenclature:: 0 (Genetic Markers)
Entry Date(s):: Date Created: 19940301 Date Completed: 19940729 Latest Revision: 20190904
Update Code:: 20240104
DOI:: 10.1006/geno.1994.1136
PMID:: 8020938
: Czasopismo naukowe

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Two somatic cell hybrids containing the der(12) or the der(X) from a mesothelioma with a translocation t(X;12)(q22;p13) as the only chromosomal change were generated to characterize the region of 12p13 containing the translocation breakpoint. Fluorescence in situ hybridization analysis showed the breakpoint on chromosome 12 to occur between VWF and D12S158. On the linkage map developed by J. Weissenbach et al. (Nature, 1992, 359: 794-801), the breakpoints were located between D12S99 and D12S100 on chromosome 12 and between DXS1106 and DXS1001 on chromosome X. PCR analysis based on genomic sequences, with DNA from both somatic cell hybrids, enabled us to map CACNL1A1, FGF6, D12S370, D12S38OE, D12S381E, and D12S382E distally to the 12p13 breakpoint and to VWF.

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