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Tytuł pozycji:

GCN4p activation of the yeast TRP3 gene is enhanced by ABF1p and uses a suboptimal TATA element.

Tytuł:
GCN4p activation of the yeast TRP3 gene is enhanced by ABF1p and uses a suboptimal TATA element.
Autorzy:
Martens JA; Department of Biochemistry, University of Western Ontario, London, Canada.
Brandl CJ
Źródło:
The Journal of biological chemistry [J Biol Chem] 1994 Jun 03; Vol. 269 (22), pp. 15661-7.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology
Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology
MeSH Terms:
Gene Expression Regulation, Fungal*
Genes, Fungal*
Saccharomyces cerevisiae Proteins*
TATA Box*
Transcription Factors*
Anthranilate Synthase/*biosynthesis
DNA-Binding Proteins/*metabolism
Fungal Proteins/*metabolism
Indole-3-Glycerol-Phosphate Synthase/*biosynthesis
Multienzyme Complexes/*biosynthesis
Protein Kinases/*metabolism
Saccharomyces cerevisiae/*genetics
Anthranilate Synthase/genetics ; Base Sequence ; Binding Sites ; DNA, Fungal/metabolism ; Indole-3-Glycerol-Phosphate Synthase/genetics ; Molecular Sequence Data ; Multienzyme Complexes/genetics ; Mutagenesis, Site-Directed ; Oligodeoxyribonucleotides ; Point Mutation ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae/metabolism ; Transcription, Genetic
Gene Symbol:
TRP3
Substance Nomenclature:
0 (ABF1 protein, S cerevisiae)
0 (DNA, Fungal)
0 (DNA-Binding Proteins)
0 (Fungal Proteins)
0 (Multienzyme Complexes)
0 (Oligodeoxyribonucleotides)
0 (Saccharomyces cerevisiae Proteins)
0 (Transcription Factors)
EC 2.7.- (Protein Kinases)
EC 4.1.1.48 (Indole-3-Glycerol-Phosphate Synthase)
EC 4.1.3.27 (Anthranilate Synthase)
EC 4.1.3.27 (TRP3 protein, S cerevisiae)
Entry Date(s):
Date Created: 19940603 Date Completed: 19940630 Latest Revision: 20210210
Update Code:
20240104
PMID:
8195216
Czasopismo naukowe
Transcription of the TRP3 gene of Saccharomyces cerevisiae is regulated by GCN4p from a position proximal to the transcriptional initiation sites. The promoter's apparent lack of a conventional TATA element sequence has led it to be used as a model for TATA-less promoters. Through mutational analysis of the TRP3 promoter, we have identified two additional regulatory elements required for expression. The first, located 57 base pairs (bp) upstream of the GCN4p binding site, binds ABF1p in vitro. The ABF1p binding site was required for maximal levels of GCN4p-activated transcription in vivo; however, the -fold activation by GCN4p was not altered by ABF1p. The second element, positioned 23 bp downstream of the GCN4p binding site, contained the TATA-like sequence, TATTAA. This element was required for both basal and activated expression and almost certainly functions as a TATA-binding protein interaction site. Mutations that improved its TATA character for native or an altered specificity mutant of TATA-binding protein correspondingly improved its function. Interestingly, basal expression induced by ABF1p was virtually unchanged in the presence of point mutations in the TATTAA element. Furthermore, unlike the case for HIS3 where only a limited subset of TATA-like sequences can activate transcription in conjunction with GCN4p, many divergent TATA-like sequences allowed GCN4p activation of TRP3. We suggest that the apparent promoter specific use of these TATA elements by GCN4p results from ABF1p amplifying the GCN4p-induced expression to a detectable level.

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