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Tytuł:
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Interaction of ETS-1 and ERGB/FLI-1 proteins with DNA is modulated by spacing between multiple binding sites as well as phosphorylation.
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Autorzy:
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Hodge DR; Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21702-1201, USA.
Robinson L
Watson D
Lautenberger J
Zhang XK
Venanzoni M
Seth A
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Źródło:
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Oncogene [Oncogene] 1996 Jan 04; Vol. 12 (1), pp. 11-8.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Publication: <2002->: Basingstoke : Nature Publishing Group
Original Publication: Basingstoke, Hampshire, UK : Scientific & Medical Division, MacMillan Press, c1987-
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MeSH Terms:
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DNA/*metabolism
DNA-Binding Proteins/*metabolism
Proto-Oncogene Proteins/*metabolism
Trans-Activators/*metabolism
Transcription Factors/*metabolism
Animals ; Base Sequence ; Binding Sites ; Enhancer Elements, Genetic ; HIV Long Terminal Repeat ; Molecular Sequence Data ; Phosphorylation ; Proto-Oncogene Protein c-ets-1 ; Proto-Oncogene Protein c-fli-1 ; Proto-Oncogene Proteins c-ets ; Spodoptera
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Substance Nomenclature:
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0 (DNA-Binding Proteins)
0 (Proto-Oncogene Protein c-ets-1)
0 (Proto-Oncogene Protein c-fli-1)
0 (Proto-Oncogene Proteins)
0 (Proto-Oncogene Proteins c-ets)
0 (Trans-Activators)
0 (Transcription Factors)
9007-49-2 (DNA)
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Entry Date(s):
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Date Created: 19960104 Date Completed: 19960220 Latest Revision: 20081121
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Update Code:
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20240104
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PMID:
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8552380
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ETS is a family of transcription factors that contain a highly conserved ETS DNA binding domain. Various members of the ETS family are expressed in cells of hematopoietic lineage. ETS-1, ETS-2 and ERGB/FLI-1 are expressed at high levels in T-lymphocytes. HIV-1 infects T-cells and it has been shown that its LTR contains binding sites for various transcription factors. In this study we show that the HIV-1 core enhancer is directly regulated by ERGB/FLI-1 protein positively, as well as, negatively, depending upon the presence or absence of accessory factors in different cell types. In addition, we show that the ETS-1 transactivation activity is enhanced upon dephosphorylation of the Calmodulin-dependent Protein Kinase II phosphorylation site located in exon VII. Finally, we demonstrate that the spacing between the two EBS cores in palindromic or direct repeat sites play a crucial role in binding of ETS proteins to DNA.