Heterogeneic modulation of malignant behavior in human glioma cells in defined and serum-containing media.

Szczegóły
Abstrakt

Tytuł:: Heterogeneic modulation of malignant behavior in human glioma cells in defined and serum-containing media.
Autorzy:: Haugland HK; Department of Pathology, Gade Institute, University of Bergen, Norway.
Tysnes OB
Źródło:: In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 1996 Mar; Vol. 32 (3), pp. 159-66.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: Berlin : Springer
Original Publication: Columbia, MD : Tissue Culture Association, c1993-
MeSH Terms:: Brain Neoplasms*/chemistry
Brain Neoplasms*/pathology
Cell Culture Techniques*
Culture Media*
Glioma*/chemistry
Cell Division ; Cell Movement ; Flow Cytometry ; Humans ; Neoplasm Invasiveness ; Spheroids, Cellular ; Tumor Cells, Cultured
References:: Pharmacol Ther. 1992;53(3):355-74. (PMID: 1409851)
Cancer Res. 1986 Aug;46(8):4071-9. (PMID: 3731074)
Cancer Lett. 1988 Jan;38(3):283-96. (PMID: 3258178)
Science. 1977 Aug 19;197(4305):776-8. (PMID: 302030)
Cancer Res. 1978 Oct;38(10):3333-9. (PMID: 688223)
J Cell Biol. 1980 Nov;87(2 Pt 1):434-41. (PMID: 7000795)
J Neurosurg. 1990 Jul;73(1):106-12. (PMID: 2352010)
Anticancer Res. 1988 Jul-Aug;8(4):797-803. (PMID: 3178168)
Cancer Res. 1994 Jul 15;54(14):3897-904. (PMID: 8033113)
Anticancer Res. 1983 May-Jun;3(3):187-94. (PMID: 6870201)
Biotechnology. 1991;17:73-106. (PMID: 2049552)
Int J Cancer. 1994 Jan 15;56(2):255-61. (PMID: 8314309)
Arzneimittelforschung. 1971 Aug;21(8):1274-8. (PMID: 5110034)
Anticancer Res. 1993 Sep-Oct;13(5A):1325-30. (PMID: 8239502)
J Neurosurg. 1994 Mar;80(3):515-9. (PMID: 8113864)
Cancer Treat Rep. 1981;65 Suppl 2:67-70. (PMID: 7346149)
Anticancer Res. 1989 Mar-Apr;9(2):413-20. (PMID: 2751265)
J Neurosurg. 1990 Jul;73(1):98-105. (PMID: 2161913)
Cancer Res. 1984 Jan;44(1):254-8. (PMID: 6690037)
Neurotoxicology. 1993 Spring;14(1):19-22. (PMID: 8361674)
Invasion Metastasis. 1990;10(2):113-28. (PMID: 2307561)
In Vitro Cell Dev Biol. 1986 Apr;22(4):180-92. (PMID: 3516971)
Int J Cancer. 1983 May 15;31(5):523-33. (PMID: 6852971)
J Neurosci Res. 1982;7(2):215-28. (PMID: 6212693)
J Neuropathol Exp Neurol. 1981 May;40(3):201-29. (PMID: 6260907)
Cancer Res. 1977 Oct;37(10):3639-43. (PMID: 908012)
In Vitro Cell Dev Biol. 1986 Apr;22(4):193-200. (PMID: 3700322)
Biol Cell. 1988;63(3):263-8. (PMID: 3066424)
Substance Nomenclature:: 0 (Culture Media)
Entry Date(s):: Date Created: 19960301 Date Completed: 19961025 Latest Revision: 20191101
Update Code:: 20240104
DOI:: 10.1007/BF02723681
PMID:: 8925138
: Czasopismo naukowe

Malignant features in three glioma cell lines were studied in four defined media of various complexity. The cell lines D37MG, D54MG, and GaMG were able to grow in monolayer culture in all media examined, and as multicellular tumor spheroids in the two most nutrient-rich media. In the defined media, none of the cell lines were able to migrate in a migration assay on poly-D-lysine-coated plastic surfaces. Flow cytometric analysis of the GaMG cell line demonstrated no medium-dependent selection of subclones of glioma cells in spheroids cultured for 30 d. Morphological diversity of spheroids varied according to the supplementation of the media. The capacity of glioma cells to invade cellular rat brain aggregates was intact in the media examined. However, glioma migration was severely inhibited by the lack of specific serum components. This study demonstrates that glioma growth and invasion was heterogeneously preserved in the defined media used. Depending on the assay to be used in the study of glioma cell behavior, the degree of medium supplementation has to be considered.

Informacja