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Tytuł:
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Insulin does not stimulate β-alanine transport into human skeletal muscle.
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Autorzy:
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de Souza Gonçalves, Lívia
Kratz, Caroline
Santos, Lívia
Henrique Carvalho, Victor
Peixoto Sales, Lucas
Nemezio, Kleiner
Longobardi, Igor
Augusto Riani, Luiz
de Oliveira Lima, Marcelo Miranda
Saito, Tiemi
Lins Fernandes, Alan
Rodrigues, Joice
Margaret James, Ruth
Sale, Craig
Gualano, Bruno
Geloneze, Bruno
Gennari de Medeiros, Marisa Helena
Giannini Artioli, Guilherme
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Temat:
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SKELETAL muscle
INSULIN
MUSCLE cells
HYPERINSULINISM
CARNOSINE
BLOOD sampling
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Źródło:
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American Journal of Physiology: Cell Physiology; Apr2020, Vol. 318 Issue 4, pC777-C786, 10p
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To test whether high circulating insulin concentrations influence the transport of β-alanine into skeletal muscle at either saturating or subsaturating β-alanine concentrations, we conducted two experiments whereby β-alanine and insulin concentrations were controlled. In experiment 1, 12 men received supraphysiological amounts of β-alanine intravenously (0.11 g·kg·1·min·1 for 150 min), with or without insulin infusion. β-Alanine and carnosine were measured in muscle before and 30 min after infusion. Blood samples were taken throughout the infusion protocol for plasma insulin and β-alanine analyses. β-Alanine content in 24-h urine was assessed. In experiment 2, six men ingested typical doses of β-alanine (10 mg/kg) before insulin infusion or no infusion. β-Alanine was assessed in muscle before and 120 min following ingestion. In experiment 1, no differences between conditions were shown for plasma β-alanine, muscle β-alanine, muscle carnosine and urinary β-alanine concentrations (all P > 0.05). In experiment 2, no differences between conditions were shown for plasma β-alanine or muscle β-alanine concentrations (all P > 0.05). Hyperinsulinemia did not increase β-alanine uptake by skeletal muscle cells, neither when substrate concentrations exceed the Vmax of β-alanine transporter TauT nor when it was below saturation. These results suggest that increasing insulin concentration is not necessary to maximize β-alanine transport into muscle following β-alanine intake. [ABSTRACT FROM AUTHOR]
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