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Tytuł:
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Sickle Cell Disease: Metabolomic Profiles of Vaso-Occlusive Crisis in Plasma and Erythrocytes.
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Autorzy:
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Dembélé, Klétigui Casimir
Veyrat-Durebex, Charlotte
Guindo, Aldiouma
Chupin, Stéphanie
Tessier, Lydie
Goïta, Yaya
Baraïka, Mohamed Ag
Diallo, Moussa
Touré, Boubacari Ali
Homedan, Chadi
Mirebeau-Prunier, Delphine
Simard, Gilles
Diallo, Dapa
Cissé, Bakary Mamadou
Reynier, Pascal
Chao de la Barca, Juan Manuel
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Temat:
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SICKLE cell anemia
ERYTHROCYTES
RECEIVER operating characteristic curves
DISCRIMINANT analysis
LEAST squares
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Źródło:
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Journal of Clinical Medicine; Apr2020, Vol. 9 Issue 4, p1092, 1p
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The metabolomic profile of vaso-occlusive crisis, compared to the basal state of sickle cell disease, has never been reported to our knowledge. Using a standardized targeted metabolomic approach, performed on plasma and erythrocyte fractions, we compared these two states of the disease in the same group of 40 patients. Among the 188 metabolites analyzed, 153 were accurately measured in plasma and 143 in red blood cells. Supervised paired partial least squares discriminant analysis (pPLS-DA) showed good predictive performance for test sets with median area under the receiver operating characteristic (AUROC) curves of 99% and mean p-values of 0.0005 and 0.0002 in plasma and erythrocytes, respectively. A total of 63 metabolites allowed discrimination between the two groups in the plasma, whereas 61 allowed discrimination in the erythrocytes. Overall, this signature points to altered arginine and nitric oxide metabolism, pain pathophysiology, hypoxia and energetic crisis, and membrane remodeling of red blood cells. It also revealed the alteration of metabolite concentrations that had not been previously associated with sickle cell disease. Our results demonstrate that the vaso-occlusive crisis has a specific metabolomic signature, distinct from that observed at steady state, which may be potentially helpful for finding predictive biomarkers for this acute life-threatening episode. [ABSTRACT FROM AUTHOR]
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