Bioaerosol sampling is a critical step toward characterizing airborne pathogen exposure risks for many respiratory infectious diseases in human-to-human or human-to-animal outbreaks. The integrated performance-parameter design and sampling efficiency of aerosol-to-hydrosol samplers (cyclones) has been rarely published or studied. This review focuses on the determination of cyclone performance-parameter design and impact factors of the sampling efficiency, discusses their mechanisms of collection and describes their advantages and drawbacks. The crucial design parameters of cyclones in different applications are then discussed in detail. We also discuss how the aerosol-to-hydrosol sampling efficiency is intrinsically related to the performance parameters for bioaerosol cyclone design. In the final section of this review, scientific questions and challenges for future work are presented to encourage high collection efficiency design and high-quality cyclones. [ABSTRACT FROM AUTHOR]
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