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Title of the item:

猴面包树的离体快繁条件筛选. (Chinese)

Title :
猴面包树的离体快繁条件筛选. (Chinese)
Authors :
刘 扬
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Subject Terms :
ADANSONIA digitata
ROOTING of plant cuttings
TISSUE culture
GERMPLASM conservation
PLANT shoots
Alternate Title :
Screening for in vitro rapid propagation conditions of Adansonia digitata. (English)
Source :
Guihaia; feb2021, Vol. 41 Issue 2, p292-300, 9p
Abstract (English) :
The purpose of this paper was to establish the tissue culture regeneration system by using the seeds of Adansonia digitata. After treated with a suitable pretreatment and seeds sterilizing method, the initial medium was inoculated to obtain a sterile material. After initiation culture, the multiple shoot were induced in the enrichment medium, and then cut into individual plants for rooting induction, and finally established a tissue culture and rapid propagation system. The results were as follows: The optimal disinfection treatment combination of explants was 75% alcohol treatment for 3 min + 0.1% HgCl, treatment for 15 min, the contamination rate of explants was 21.7%; The germination rate was 46.0% after pretreatment with 95% concentrated sulfuric acid for 12 h; The A. digitata seeds were pretreated and sterilized and inoculated onto WPM start-up medium for 48 h, and the germination rate was 74.0%; After the initial culture, axillary buds of the explants were cut into 2-3 cm with a section of young stems and inoculated into the enrichment medium and cultured for 60 d for multiple shoots induction, finally, the best value-added medium was selected as WPMBA, and rooting was induced for 60 d with a rooting rate of 61.3%, the average number of roots was 1.9 and the average root length was 8.9 cm. Adding the right amount of IBA can improve the foam root phenomenon and improve the transplant survival rate. After seedling adaptation, the tissue culture seedlings of A. digitata were transplanted to mixed matrix with coconut vegetable garden soil perlite (volume ratio 1:1:1), and the survival rate reached above 60.0%. This paper preliminarily established the in titro rapid propagation technology system of A. digitata, and can provide theoretical and technical supports for the rapid propagation of A. digitata and the preservation of germplasm resources. [ABSTRACT FROM AUTHOR]
Academic Journal
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