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Tytuł pozycji:

Continuous culture and synchronization of Hyphomicrobium sp. B-522.

Tytuł :
Continuous culture and synchronization of Hyphomicrobium sp. B-522.
Autorzy :
Matzen, N.
Hirsch, P.
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Źródło :
Archives of Microbiology; Jul1982, Vol. 132 Issue 1, p96-99, 4p
Czasopismo naukowe
A technique was developed for synchronization of Hyphomicrobium sp. strain B-522. Bacteria were grown in continuous culture with methanol (0.1%; v/v) growth limiting. Vitamin B (2.5 μg/l) was necessary to obtain steady state growth. The critical dilution rate was D=0.112; maximum cell output occurred at D=0.105 ( Dx=30 mg l h). Continuous cultures of Hyphomicrobium B-522 at D=0.110 were used to obtain cells for synchronization experiments. Synchronization was achieved by trapping young hyphal and budding cells in a glass wool column, while the initial swarmer cells were allowed to pass through. By semicontinuously rinsing the system, newly produced swarmers could be sampled in the effluent. The mean length of these synchronous swarmer cells was 1.25 μm ( s=±0.13 μm; range 0,6 μm) as compared to 1.40 μm ( s=±0.21 μm; range 1.2 μm) for swarmer cells of the continuous culture. Division of synchronous swarmer populations was completed after 7 h; the synchronization index was 0.76. [ABSTRACT FROM AUTHOR]
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