Mise au point et validation d'une méthode de dosage de l'amphotéricine B par chromatographie liquide à haute performance. (French)

Szczegóły
Abstrakt

Tytuł:: Mise au point et validation d'une méthode de dosage de l'amphotéricine B par chromatographie liquide à haute performance. (French)
Autorzy:: Gaïes, Emna
Ben Ali, Ridha
Trabelsi, Sameh
Jebabli, Nadia
Salouage, Issam
Charfi, Rim
Lakhal, Mohamed
Klouz, Anis
Temat:: MYCOSIS fungoides
AMPHOTERICIN B
HIGH performance liquid chromatography
ANTIFUNGAL agents
CHROMATOGRAPHIC analysis
ULTRAVIOLET detectors
THERAPEUTICS
Alternatywny tytuł:: Monitoring and validation of amphotericin B by HPLC (English)
Źródło:: Revue Francophone des Laboratoires; Feb2013 Part 1, Vol. 2013 Issue 449, p67-70, 4p
Abstract (English):: Summary: Amphotericin B is an effective antifungal drug used in the treatment of systemic mycosis, but it has a narrow therapeutic interval and an important renal toxicity. The aim of this study is to monitor and valid a new method for Amphotericin B quantitation in plasma using HPLC with UV/visible detection. A liquid-liquid extraction was done; thereafter, a chromatographic separation was achieved using an RP 18 column (125mm). The mobile phase is composed of water, acetonitrile and acetic acid (51/44/5) (v/v/v), pH 3. Piroxicam is used as an internal standard. This technique is linear over the range 0,125μg/mL to 8μg/mL (r=0,99). Detection and quantification limits were 0.044μg/mL and 0.111μg/mL, respectively. Within-day coefficient of variation (1,98 to 4,66 %) and day-to-day coefficient of variation (2,69 to 4,95 %) were determined at three different concentrations. Under these conditions, each analysis requires no longer than 10min. [ABSTRACT FROM AUTHOR]
: Czasopismo naukowe

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