Syntheses of AZ12320927 labeled with H3, C14, and H2

In support of a program to develop a treatment for depression, three isotopically labeled forms of the 5HT1Bantagonist AZ12320927 were synthesized. A tritium labeled version was synthesized for autoradiography using Ircatalyzed hydrogen–tritium exchange. A C14 labeled version was prepared for use in metabolism studies in foursteps from u14Cpnitrophenol. A stable isotope labeled version was synthesized for use as an internal standard for LCMSMS quantitation in two steps from chromenone 1. Copyright © 2008 John Wiley & Sons, Ltd.