-
Tytuł:
-
New plate medium for screening and presumptive identification of gram-negative urinary tract pathogens
-
Autorzy:
-
Thaller, M C
Berlutti, F
Dainelli, B
Pezzi, R
-
Źródło:
-
Journal of Clinical Microbiology; April 1988, Vol. 26 Issue: 4 p791-793, 3p
-
A new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. The medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucuronidase tests and allows the indole and cytochrome oxidase tests to be performed directly from the plates. High-level agreement with individual conventional tests was recorded in comparative studies with 504 cultures of gram-negative rods. There was 100% agreement, except for the Providencia spp. indole spot test (61.6% agreement). Adonitol fermentation by Providencia species could not be determined. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa were identified with a high efficiency (100, 85.7, 83.5, and 100% agreement, respectively) without further testing. There was 96% overall agreement for the 267 infected urine samples tested.