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Title of the item:

β-Glucuronidase-Cleavable Prodrugs of O6-Benzylguanine and O6-Benzyl-2‘-deoxyguanosine

Title :
β-Glucuronidase-Cleavable Prodrugs of O6-Benzylguanine and O6-Benzyl-2‘-deoxyguanosine
Authors :
Wei, G.
Loktionova, N. A.
Pegg, A. E.
Moschel, R. C.
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Source :
Journal of Medicinal Chemistry; January 2005, Vol. 48 Issue: 1 p256-261, 6p
Periodical
Glucuronic acid linked prodrugs of O6-benzylguanine and O6-benzyl-2‘-deoxyguanosine were synthesized. The prodrugs were found to be quite stable at physiological pH and were more than 200-fold less active as inactivators of O6-alkylguanine-DNA alkyltransferase (alkyltransferase) than either O6-benzylguanine or O6-benzyl-2‘-deoxyguanosine. β-Glucuronidase from both Escherichia coli and bovine liver cleaved the prodrugs efficiently to release O6-benzylguanine and O6-benzyl-2‘-deoxyguanosine, respectively. In combination with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), the prodrugs were not effective adjuvants for HT29 cell killing. However, as expected, incubation of these prodrugs with β-glucuronidase in the culture medium led to much more efficient cell killing by BCNU as a result of the liberation of the more potent inactivators, O6-benzylguanine and O6-benzyl-2‘-deoxyguanosine. These prodrugs may be useful for prodrug monotherapy of necrotic tumors that liberate β-glucuronidase or for antibody-directed enzyme prodrug therapy with antibodies that can deliver β-glucuronidase to target tumor cells.

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