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Tytuł pozycji:

Geneva Cocktail for Cytochrome P450 and P-glycoprotein Activity Assessment Using Dried Blood Spots

Tytuł :
Geneva Cocktail for Cytochrome P450 and P-glycoprotein Activity Assessment Using Dried Blood Spots
Autorzy :
Bosilkovska, M.
Samer, C.F.
Déglon, J.
Rebsamen, M.
Staub, C.
Dayer, P.
Walder, B.
Desmeules, J.A.
Daali, Y.
Pokaż więcej
Temat :
ddc:617
ddc:614.1
Administration, Oral
Adult
Bupropion/administration & dosage
Bupropion/blood
Caffeine/administration & dosage
Caffeine/blood
Capsules
Carbonated Beverages
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Coffee
Cytochrome P-450 Enzyme System/antagonists & inhibitors
Cytochrome P-450 Enzyme System/blood
Dextromethorphan/administration & dosage
Dextromethorphan/blood
Dried Blood Spot Testing
Enzyme Inhibitors/administration & dosage
Feasibility Studies
Flurbiprofen/administration & dosage
Flurbiprofen/blood
Humans
Isoenzymes
Male
Midazolam/administration & dosage
Midazolam/blood
Omeprazole/administration & dosage
Omeprazole/blood
P-Glycoprotein/antagonists & inhibitors
P-Glycoprotein/blood
Pharmaceutical Preparations/administration & dosage
Pharmaceutical Preparations/blood
Phenotype
Pilot Projects
Predictive Value of Tests
Spectrometry, Mass, Electrospray Ionization
Substrate Specificity
Tandem Mass Spectrometry
Terfenadine/administration & dosage
Terfenadine/analogs & derivatives
Young Adult
Articles
ddc:576
Źródło :
Clinical Pharmacology and Therapeutics
Clinical Pharmacology and Therapeutics, Vol. 96, No 3 (2014) pp. 349-359
Clinical Pharmacology and Therapeutics, vol. 96, no. 3, pp. 349-359
Wydawca :
Nature Publishing Group, 2014.
Rok publikacji :
2014
Oryginalny identyfikator :
pmc: PMC4151019
pmid: 24722393
Opis pliku :
application/pdf
Język :
English
ISSN :
1532-6535
0009-9236
DOI :
10.1038/clpt.2014.83
The suitability of the capillary dried blood spot (DBS) sampling method was assessed for simultaneous phenotyping of cytochrome P450 (CYP) enzymes and P-glycoprotein (P-gp) using a cocktail approach. Ten volunteers received an oral cocktail capsule containing low doses of the probes bupropion (CYP2B6), flurbiprofen (CYP2C9), omeprazole (CYP2C19), dextromethorphan (CYP2D6), midazolam (CYP3A), and fexofenadine (P-gp) with coffee/Coke (CYP1A2) on four occasions. They received the cocktail alone (session 1), and with the CYP inhibitors fluvoxamine and voriconazole (session 2) and quinidine (session 3). In session 4, subjects received the cocktail after a 7-day pretreatment with the inducer rifampicin. The concentrations of probes/metabolites were determined in DBS and plasma using a single liquid chromatography-tandem mass spectrometry method. The pharmacokinetic profiles of the drugs were comparable in DBS and plasma. Important modulation of CYP and P-gp activities was observed in the presence of inhibitors and the inducer. Minimally invasive one- and three-point (at 2, 3, and 6 h) DBS-sampling methods were found to reliably reflect CYP and P-gp activities at each session.Clinical Pharmacology & Therapeutics (2014); advance online publication 7 May 2014. doi:10.1038/clpt.2014.83.

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