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Tytuł pozycji:

De novo transcriptome analysis of Medicago falcata reveals novel insights about the mechanisms underlying abiotic stress-responsive pathway

Tytuł :
De novo transcriptome analysis of Medicago falcata reveals novel insights about the mechanisms underlying abiotic stress-responsive pathway
Autorzy :
Miao, Zhenyan
Xu, Wei
Li, Daofeng
Hu, Xiaona
Liu, Jiaxing
Zhang, Rongxue
Tong, Zongyong
Dong, Jiangli
Su, Zhen
Zhang, Liwei
Sun, Min
Li, Wenjie
Du, Zhenglin
Hu, Songnian
Wang, Tao
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Temat :
food and beverages
Biotechnology
Research Article
Genetics
Legume
Transcriptome
Medicago falcata
Nodule
Phytohormones
Źródło :
BMC Genomics. 16
Wydawca :
Springer Nature, 2015.
Rok publikacji :
2015
Oryginalny identyfikator :
pmc: PMC4615886
pmid: 26481731
Język :
English
ISSN :
1471-2164
DOI :
10.1186/s12864-015-2019-x
Background The entire world is facing a deteriorating environment. Understanding the mechanisms underlying plant responses to external abiotic stresses is important for breeding stress-tolerant crops and herbages. Phytohormones play critical regulatory roles in plants in the response to external and internal cues to regulate growth and development. Medicago falcata is one of the stress-tolerant candidate leguminous species and is able to fix atmospheric nitrogen. This ability allows leguminous plants to grow in nitrogen deficient soils. Methods We performed Illumina sequencing of cDNA prepared from abiotic stress treated M. falcata. Sequencedreads were assembled to provide a transcriptome resource. Transcripts were annotated using BLASTsearches against the NCBI non-redundant database and gene ontology definitions were assigned. Acomparison among the three abiotic stress treated samples was carried out. The expression of transcriptswas confirmed with qRT-PCR. Results We present an abiotic stress-responsive M. falcata transcriptome using next-generation sequencing data from samples grown under standard, dehydration, high salinity, and cold conditions. We combined reads from all samples and de novo assembled 98,515 transcripts to build the M. falcata gene index. A comprehensive analysis of the transcriptome revealed abiotic stress-responsive mechanisms underlying the metabolism and core signalling components of major phytohormones. We identified nod factor signalling pathways during early symbiotic nodulation that are modified by abiotic stresses. Additionally, a global comparison of homology between the M. falcata and M. truncatula transcriptomes, along with five other leguminous species, revealed a high level of global sequence conservation within the family. Conclusions M. falcata is shown to be a model candidate for studying abiotic stress-responsive mechanisms in legumes. This global gene expression analysis provides new insights into the biochemical and molecular mechanisms involved in the acclimation to abiotic stresses. Our data provides many gene candidates that might be used for herbage and crop breeding. Additionally, FalcataBase (http://bioinformatics.cau.edu.cn/falcata/) was built for storing these data. Electronic supplementary material The online version of this article (doi:10.1186/s12864-015-2019-x) contains supplementary material, which is available to authorized users.

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