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Title of the item:

TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells

Title :
TET1 exerts its anti-tumor functions via demethylating DACT2 and SFRP2 to antagonize Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma cells
Authors :
Fan, Jiangxia
Zhang, Yan
Mu, Junhao
He, Xiaoqian
Shao, Bianfei
Zhou, Dishu
Peng, Weiyan
Tang, Jun
Jiang, Yu
Ren, Guosheng
Xiang, Tingxiu
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Subject Terms :
Demethylation
Nasopharyngeal carcinoma
QH426-470
Genetics
TET1
Tumor suppressor
Medicine
Research
Wnt pathway
Source :
Clinical Epigenetics, Vol 10, Iss 1, Pp 1-16 (2018)
Publisher :
BMC, 2018.
Publication Year :
2018
Original Identifier :
pmc: PMC6091063
pmid: 30075814
Language :
English
ISSN :
1868-7083
1868-7075
DOI :
10.1186/s13148-018-0535-7
Background TET1 is a tumor suppressor gene (TSG) that codes for ten-eleven translocation methyl cytosine dioxygenase1 (TET1) catalyzing the conversion of 5-methylcytosine to 5-hydroxy methyl cytosine as a first step of TSG demethylation. Its hypermethylation has been associated with cancer pathogenesis. However, whether TET1 plays any role in nasopharyngeal carcinoma (NPC) remains unclear. This study investigated the expression and methylation of TET1 in NPC and confirmed its role and mechanism as a TSG. Results TET1 expression was downregulated in NPC tissues compared with nasal septum deviation tissues. Demethylation of TET1 in HONE1 and HNE1 cells restored its expression with downregulated methylation, implying that TET1 was silenced by promoter hypermethylation. Ectopic expression of TET1 suppressed the growth of NPC cells, induced apoptosis, arrested cell division in G0/G1 phase, and inhibited cell migration and invasion, confirming TET1 TSG activity. TET1 decreased the expression of nuclear β-catenin and downstream target genes. Furthermore, TET1 could cause Wnt antagonists (DACT2, SFRP2) promoter demethylation and restore its expression in NPC cells. Conclusions Collectively, we conclude that TET1 exerts its anti-tumor functions in NPC cells by suppressing Wnt/β-catenin signaling via demethylation of Wnt antagonists (DACT2 and SFRP2). Electronic supplementary material The online version of this article (10.1186/s13148-018-0535-7) contains supplementary material, which is available to authorized users.
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