Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ
Tytuł pozycji:

Two conformationally distinct α-synuclein oligomers share common epitopes and the ability to impair long-term potentiation

Tytuł :
Two conformationally distinct α-synuclein oligomers share common epitopes and the ability to impair long-term potentiation
Autorzy :
Otzen, Daniel Erik
van Diggelen, Femke
Hrle, Dean
Apetri, Mihaela
Christiansen, Gunna
Rammes, Gerhard
Tepper, Armand
Pokaż więcej
Temat :
Toxicity
Research Article
Monomers
Anatomy
Oligomers
Pathology and Laboratory Medicine
Polymer Chemistry
NEURONS
Vesicles
SECONDARY STRUCTURE
Neurodegenerative Diseases
Physical Sciences
TRANSMISSION
Electron Microscopy
Transmission Electron Microscopy
SYNAPTIC DYSFUNCTION
MECHANISM
Neurology
Chemistry
Toxicology
ANTIPARALLEL BETA-SHEET
Cellular Types
LEWY BODY
Biology and Life Sciences
AMYLOID OLIGOMERS
Materials Science
Neuroscience
Research and Analysis Methods
Physiology
Animal Cells
Medicine
Parkinson Disease
Cellular Neuroscience
Cell Biology
Nervous System
Synapses
Electrophysiology
Materials
Cellular Structures and Organelles
Science
FATTY-ACIDS
Microscopy
Medicine and Health Sciences
Movement Disorders
Neurophysiology
OXIDATIVE STRESS
Źródło :
PLoS ONE, Vol 14, Iss 3, p e0213663 (2019)
van Diggelen, F, Hrle, D, Apetri, M, Christiansen, G, Rammes, G, Tepper, A & Otzen, D E 2019, ' Two conformationally distinct α-synuclein oligomers share common epitopes and the ability to impair long-term potentiation ', PLOS ONE, vol. 14, no. 3, 0213663 . https://doi.org/10.1371/journal.pone.0213663
Wydawca :
Public Library of Science (PLoS), 2019.
Rok publikacji :
2019
Kolekcja :
PURE_Aarhus_University
DOAJ-Articles
Oryginalny identyfikator :
pmc: PMC6430514
pmid: 30901378
Język :
English
ISSN :
1932-6203
DOI :
10.1371/journal.pone.0213663
Parkinson's Disease (PD) is a neurodegenerative disease for which there currently is no cure. Aggregation of the pre-synaptic protein α-synuclein (aSN) into oligomers (αSOs) is believed to play a key role in PD pathology, but little is known about αSO formation in vivo and how they induce neurodegeneration. Both the naturally occurring polyunsaturated fatty acid docosahexaenoic acid (DHA) and the lipid peroxidation product 4-hydroxynonenal (HNE), strongly upregulated during ROS conditions, stimulate the formation of αSOs, highlighting a potential role in PD. Yet, insight into αSOs structure and biological effects is still limited as most oligomer preparations studied to date are heterogeneous in composition. Here we have aggregated aSN in the presence of HNE and DHA and purified the αSOs using size exclusion chromatography. Both compounds stimulate formation of spherical αSOs containing anti-parallel β-sheet structure which have the same shape as unmodified αSOs though ca. 2-fold larger. Furthermore, the yield and stabilities of these oligomers are significantly higher than for unmodified aSN. Both modified and unmodified αSOs permeabilize synthetic vesicles, show high co-localisation with glutamatergic synapses and decrease Long Term Potentiation (LTP), in line with the reported synaptotoxic effects of αSOs. We conclude that DHA- and HNE-αSOs are convenient models for pathogenic disease-associated αSOs in PD.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies