Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ
Tytuł pozycji:

PRMT5-mediated histone H4 arginine-3 symmetrical dimethylation marks chromatin at G + C-rich regions of the mouse genome

Tytuł :
PRMT5-mediated histone H4 arginine-3 symmetrical dimethylation marks chromatin at G + C-rich regions of the mouse genome
Autorzy :
Girardot, Michael
Hirasawa, Ryutaro
Kacem, Salim
Fritsch, L.
Pontis, J.
Kota, Satya
Filipponi, Doria
Fabbrizio, Eric
Sardet, Claude
Lohmann, F.
Kadam, S.
Ait-Si-Ali, S.
Feil, Robert
Pokaż więcej
Temat :
Animals
Mice
Genome
Cells
Cultured
Alleles
Promoter Regions
Genetic
Methylation
GC Rich Sequence
Arginine/*metabolism
Chromatin/*enzymology
DNA Methylation
Fibroblasts/enzymology
Genomic Imprinting
Histones/chemistry/*metabolism
Protein Methyltransferases/*metabolism
[SDV.CAN]Life Sciences [q-bio]/Cancer
Źródło :
Nucleic Acids Research, Oxford University Press, 2014, 42, pp.235--48. ⟨10.1093/nar/gkt884⟩
Wydawca :
Oxford University Press, 2014.
Rok publikacji :
2014
Kolekcja :
INRIA_a_CCSD_electronic_archive_server
Język :
English
ISSN :
0305-1048
1362-4962
DOI :
10.1093/nar/gkt884
Numer akcesji :
edsair.od.......165..92841da6c12cb9db57d88597e83d8ba1
Symmetrical dimethylation on arginine-3 of histone H4 (H4R3me2s) has been reported to occur at several repressed genes, but its specific regulation and genomic distribution remained unclear. Here, we show that the type-II protein arginine methyltransferase PRMT5 controls H4R3me2s in mouse embryonic fibroblasts (MEFs). In these differentiated cells, we find that the genome-wide pattern of H4R3me2s is highly similar to that in embryonic stem cells. In both the cell types, H4R3me2s peaks are detected predominantly at G + C-rich regions. Promoters are consistently marked by H4R3me2s, independently of transcriptional activity. Remarkably, H4R3me2s is mono-allelic at imprinting control regions (ICRs), at which it marks the same parental allele as H3K9me3, H4K20me3 and DNA methylation. These repressive chromatin modifications are regulated independently, however, since PRMT5-depletion in MEFs resulted in loss of H4R3me2s, without affecting H3K9me3, H4K20me3 or DNA methylation. Conversely, depletion of ESET (KMT1E) or SUV420H1/H2 (KMT5B/C) affected H3K9me3 and H4K20me3, respectively, without altering H4R3me2s at ICRs. Combined, our data indicate that PRMT5-mediated H4R3me2s uniquely marks the mammalian genome, mostly at G + C-rich regions, and independently from transcriptional activity or chromatin repression. Furthermore, comparative bioinformatics analyses suggest a putative role of PRMT5-mediated H4R3me2s in chromatin configuration in the nucleus.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies