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Tytuł pozycji:

Decreased expression of eosinophil peroxidase and major basic protein messenger RNAs during eosinophil maturation.

Tytuł :
Decreased expression of eosinophil peroxidase and major basic protein messenger RNAs during eosinophil maturation.
Autorzy :
Gruart, V
Truong, M
Plumas, J.
Zandecki, M
Kusnierz, J
Prin, L
Vinatier, D.
Capron, A.
Capron, M.
Pokaż więcej
Temat :
MESH: Actins/genetics
MESH: Base Sequence
MESH: Eosinophilia/genetics
MESH: Eosinophils/drug effects
MESH: Eosinophils/pathology
MESH: Eosinophils/physiology
MESH: Erythropoietin/genetics
MESH: Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MESH: Humans
MESH: Interleukin-3/pharmacology
MESH: Interleukin-5/pharmacology
MESH: Kinetics
MESH: Blood Proteins/genetics
MESH: Molecular Sequence Data
MESH: Neurotoxins/genetics
MESH: Oligonucleotide Probes
MESH: Peroxidases/genetics
MESH: RNA/blood
MESH: RNA/genetics
MESH: RNA/isolation & purification
MESH: RNA, Messenger/blood
MESH: RNA, Messenger/genetics
MESH: Recombinant Proteins/pharmacology
MESH: Blotting, Northern
MESH: Ribonucleases
MESH: Time Factors
MESH: Cell Differentiation/drug effects
MESH: Cells, Cultured
MESH: Eosinophil Granule Proteins
MESH: Eosinophil Peroxidase
MESH: Eosinophil-Derived Neurotoxin
MESH: Eosinophilia/blood
[SDV]Life Sciences [q-bio]
Źródło :
Blood, American Society of Hematology, 1992, 79 (10), pp.2592-7. ⟨10.1182/blood.V79.10.2592.2592⟩
Wydawca :
American Society of Hematology, 1992.
Rok publikacji :
1992
Kolekcja :
Hyper_Article_en_Ligne
Język :
English
ISSN :
0006-4971
1528-0020
DOI :
10.1182/blood.V79.10.2592.2592
Numer akcesji :
edsair.od.......166..3c2279369e18cdb32618a8437b24764e
International audience; We evaluated the levels of mRNAs encoding cationic proteins in peripheral blood eosinophils (PBE) purified from patients with eosinophilia and in eosinophils differentiated from cord blood cells (CBC) by culture with recombinant human interleukin-3 (rhIL-3), rhGM-CSF, and rhIL-5. Messenger RNAs encoding eosinophil peroxidase (EPO), major basic protein (MBP), eosinophil-derived neurotoxin (EDN), and eosinophil cationic protein (ECP) were detected by Northern blot hybridization with the respective specific oligonucleotide probes. In mature PBE, MBP mRNA appeared to be absent, whereas EPO mRNA was barely detectable in only 5 of the 19 patients. In contrast, EDN and ECP mRNAs were observed in the PBE of all patients. In CE, EPO, and MBP, mRNAs were abundant in immature eosinophils and their amounts decreased after differentiation toward eosinophils. ECP and EDN mRNAs followed the same patterns, but mRNAs were less abundant at all timepoints studied. Study of mRNA t1/2 during the time course of differentiation indicated that changes in the stability of the different mRNAs were not responsible for the variations observed in the steady-state levels. Together, these results suggest that regulation of expression differs among EPO, MBP, EDN, and ECP mRNAs during the time course of eosinophil differentiation.

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