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Tytuł pozycji:

Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca(2+) channel antagonist.

Tytuł :
Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca(2+) channel antagonist.
Autorzy :
Rousset, M
Collet, Claude
Cens, T
Bastin, F
Raymond-Delpech, Valérie
Massou, I
Menard, C
Thibaud, J-B
Charreton, Mercedes
Vignes, M
Chahine, M
Sandoz, J C
Charnet, P
Pokaż więcej
Temat :
bee
myocyte
in situ hybridization
abeille
apis mellifera
neurone récepteur
xenopus oocyte
activation cellulaire
canal calcique
hybridation in situ
[SDV]Life Sciences [q-bio]
Źródło :
Scientific Reports, Nature Publishing Group, 2017, 7, pp.41782. ⟨10.1038/srep41782⟩
Wydawca :
Nature Publishing Group, 2017.
Rok publikacji :
2017
Język :
English
ISSN :
2045-2322
DOI :
10.1038/srep41782
Numer akcesji :
edsair.od......4325..a92625e5d430608237f9af52d3ae03a0
Voltage-gated Ca(2+) channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca(2+) channel genes, grouped in 3 families (CaV1, CaV2 and CaV3), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca(2+) channels expressed in Apis mellifera. This work provides the first detailed characterization of the honeybee T-type CaV3 Ca(2+) channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca(2+) currents recorded in bee neurons and myocytes with Ca(2+) currents recorded in Xenopus oocytes expressing the honeybee CaV3 gene suggests native expression in bee muscle cells only. High-voltage activated Ca(2+) channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by in situ hybridization, immunolocalization and in vivo analysis of the effects of a CaV3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of CaV3 suggest a role in honeybee muscle function.

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