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Tytuł pozycji:

Punicalagin Inhibits Tert-Butyl Hydroperoxide-Induced Apoptosis and Extracellular Matrix Degradation in Chondrocytes by Activating Autophagy and Ameliorates Murine Osteoarthritis

Tytuł:
Punicalagin Inhibits Tert-Butyl Hydroperoxide-Induced Apoptosis and Extracellular Matrix Degradation in Chondrocytes by Activating Autophagy and Ameliorates Murine Osteoarthritis
Autorzy:
Kong J
Wang J
Gong X
Zheng X
Chen T
Temat:
punicalagin
autophagy
osteoarthritis
apoptosis
extracellular matrix
Therapeutics. Pharmacology
RM1-950
Źródło:
Drug Design, Development and Therapy, Vol Volume 14, Pp 5521-5533 (2020)
Wydawca:
Dove Medical Press, 2020.
Rok publikacji:
2020
Kolekcja:
LCC:Therapeutics. Pharmacology
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
1177-8881
Relacje:
https://www.dovepress.com/punicalagin-inhibits-tert-butyl-hydroperoxide-induced-apoptosis-and-ex-peer-reviewed-article-DDDT; https://doaj.org/toc/1177-8881
Dostęp URL:
https://doaj.org/article/d0539f44e35e45778c0bfb2466d4e832  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.0539f44e35e45778c0bfb2466d4e832
Czasopismo naukowe
Jinsong Kong, Jiacheng Wang, Xiaokang Gong, Xin Zheng, Tao Chen Department of Orthopaedics, Taizhou Municipal Hospital, Taizhou, Zhejiang, People’s Republic of ChinaCorrespondence: Tao ChenDepartment of Orthopaedics, Taizhou Municipal Hospital, Taizhou, Zhejiang, People’s Republic of ChinaEmail taizhouchentao@sina.comBackground: Osteoarthritis (OA) is a prevalent articular disorder and has no entirely satisfactory treatment. Punicalagin (PUG) is a polyphenol which has shown multiple pharmacological effects on various diseases. However, the role of PUG in the treatment of OA has not been well defined.Methods: The effects of PUG on anti-oxidative stress, anti-apoptosis, extracellular matrix (ECM) degradation and autophagy were evaluated in chondrocytes through Western blot and immunofluorescence (IF) staining. Meanwhile, the effects of PUG on destabilization of the medial meniscus (DMM) model were also assessed in vivo by performing histopathologic analysis and IF staining.Results: In vitro, PUG treatment not only increased the level of HO-1 and SOD1 against oxidative stress but also suppressed the expression of apoptotic proteins and inhibited ECM degradation. Meanwhile, PUG treatment activated autophagy and restores autophagic flux in chondrocytes after tert-butyl hydroperoxide (TBHP) insult, inhibition of autophagy by 3-methyladenine (3-MA) partly abrogated the protective effects of PUG on chondrocytes. In vivo, degeneration of the articular cartilage following DMM was also ameliorated by PUG treatment.Conclusion: PUG prevents the progression of OA through inhibition of apoptosis, oxidative stress and ECM degradation in chondrocytes, which mediated by the activation of autophagy.Keywords: punicalagin, autophagy, osteoarthritis, apoptosis, extracellular matrix

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