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Tytuł pozycji:

Mechanism of pyocyanin abolishment caused by mvaT mvaU double knockout in Pseudomonas aeruginosa PAO1

Tytuł:
Mechanism of pyocyanin abolishment caused by mvaT mvaU double knockout in Pseudomonas aeruginosa PAO1
Autorzy:
Limin Dong
Jing Pang
Xiukun Wang
Youwen Zhang
Guoqing Li
Xinxin Hu
Xinyi Yang
Chung-Dar Lu
Congran Li
Xuefu You
Temat:
pyocyanin
mvat
mvau
h-ns
quorum sensing
Infectious and parasitic diseases
RC109-216
Źródło:
Virulence, Vol 11, Iss 1, Pp 57-67 (2020)
Wydawca:
Taylor & Francis Group, 2020.
Rok publikacji:
2020
Kolekcja:
LCC:Infectious and parasitic diseases
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
2150-5594
2150-5608
21505594
Relacje:
https://doaj.org/toc/2150-5594; https://doaj.org/toc/2150-5608
DOI:
10.1080/21505594.2019.1708052
Dostęp URL:
https://doaj.org/article/ce081bb56dc649619eeeb22fbab47e6b  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.081bb56dc649619eeeb22fbab47e6b
Czasopismo naukowe
MvaT and MvaU are global transcriptional regulators belonging to the H-NS family, and pyocyanin is an important virulence factor produced by Pseudomonas aeruginosa. mvaT mvaU double knockout mutant of P. aeruginosa PAO1 demonstrated pyocyanin abolishment in the previous study. Here, we further explored the mechanism. Two main directions were studied: pyocyanin biosynthesis pathway and QS system. The effect on the expression of the pyocyanin biosynthesis genes was evaluated by promoter strength determination and Real-Time PCR assay, and significant changes leading to low pyocyanin production were found. The effect on the QS system was studied by signal molecule quantification using LC-MS/MS and related gene expression measurements using Real-Time PCR. In mvaT mvaU double knockout, the production of 3-oxo-C12-HSL obviously increased, while those of C4-HSL and PQS obviously decreased, and the changes can be recovered by mvaT or mvaU complementation. The expressions of transcriptional activator genes binding with QS system signal molecules were all decreased, resulting in decreased formation of signal-transcriptional activator complexes. And the decreased expression of rhlR and pqsE also led to the lower expression of phzA1 and phzA2. Further exploration found that QS system downregulation may be related to QsrO, a QS system repressor, which was highly upregulated with mvaT mvaU double knockout. Hence, the synthesis of pyocyanin was suffocated and the biofilm formation ability was decreased. These results were also confirmed by transcriptome analysis, which demonstrated similar gene expression changes of the aforementioned genes together with decreased expression of other virulence factor genes regulated by QS system.

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