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Tytuł pozycji:

Protective effects of Buyinqianzheng Formula on mitochondrial morphology by PINK1/Parkin pathway in SH-SY5Y cells induced by MPP+

Tytuł :
Protective effects of Buyinqianzheng Formula on mitochondrial morphology by PINK1/Parkin pathway in SH-SY5Y cells induced by MPP+
Autorzy :
Haojie Ma
Zhenyu Guo
Cong Gai
Cuicui Cheng
Jinkun Zhang
Yuxin Zhang
Luping Yang
Wandi Feng
Yushan Gao
Hongmei Sun
Pokaż więcej
Temat :
BYQZF
Parkinson’s disease
MPP+
Mitochondria
PINK1/Parkin pathway
Miscellaneous systems and treatments
RZ409.7-999
Źródło :
Journal of Traditional Chinese Medical Sciences, Vol 7, Iss 3, Pp 274-282 (2020)
Wydawca :
Elsevier, 2020.
Rok publikacji :
2020
Kolekcja :
LCC:Miscellaneous systems and treatments
Typ dokumentu :
article
Opis pliku :
electronic resource
Język :
English
ISSN :
2095-7548
Relacje :
http://www.sciencedirect.com/science/article/pii/S2095754820300697; https://doaj.org/toc/2095-7548
DOI :
10.1016/j.jtcms.2020.07.007
Dostęp URL :
https://doaj.org/article/0c86f538a533454f8217d67d342fc047
Prawa :
Journal Licence: CC BY-NC-ND
Numer akcesji :
edsdoj.0c86f538a533454f8217d67d342fc047
Czasopismo naukowe
Objective: Buyinqianzheng Formula (BYQZF) is clinically employed in traditional Chinese medicine to treat Parkinson’s disease (PD) by improving mitochondrial dysfunction. However, the underlying mechanisms by which BYQZF affects mitochondrial morphology remain unknown. Therefore, we observed the effects of BYQZF on mitochondria from the perspective of the PINK1/Parkin pathway. Methods: Cell survival rates were assessed by Cell Counting Kit-8 assay. Expression levels of PINK1 and Parkin mRNA were examined by qRT-PCR. Protein expression levels of PINK1, PINK1-Ser228, Parkin, Parkin-Ser65, Drp1, and Drp1-Ser637 were examined by western blotting. PINK1, Parkin, and MitoTracker® Red CMXRos (MTR) were stained by triple-labeled immunofluorescence, and observed under laser confocal microscopy. Results: Cell survival rate, mitochondrial form factor, mean length and number of mitochondrial network branches, mitochondrial activity, mRNA expression levels of PINK1 and Parkin, and protein expression levels of PINK1, Parkin, and Drp1-Ser637 were reduced after 1-methyl-4-phenylpyridinium (MPP+) intervention. In contrast, Pearson’s correlation coefficients between PINK1 and Parkin, and between Parkin and MTR, as well as protein expression levels of PINK1-Ser228, Parkin-Ser65, and Drp1 increased significantly after MPP+ intervention. Treatment with BYQZF increased cell survival rate, mitochondrial form factor, mean length and number of mitochondrial network branches, mitochondrial activity, mRNA expression levels of PINK1 and Parkin, and expression of PINK1, Parkin, and Drp1-Ser637 proteins. Pearson’s correlation coefficients between PINK1 and Parkin, and between Parkin and MTR, as well as protein expression levels of PINK1-Ser228, Parkin-Ser65, and Drp1 decreased after BYQZF treatment. Conclusion: These results demonstrate that BYQZF has a protective effect on mitochondrial molecular mechanisms in the PD cell model, and the mechanism is related to the PINK1/Parkin pathway.

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