AIM:To investigate the characteristic changes of ocular surface and meibomian gland function after strabismus in adolescents and analyze the related influencing factors.METHODS:Retrospective study. Totally 168 cases(224 eyes)with strabismus surgery in our hospital from January 2019 to December 2019 were selected as the study objects. According to the surgical incision, all patients were divided into the limbal incision group(group A, 54 cases, 78 eyes), trans-muscular terminal-incision group(group B, 48 cases, 62 eyes), and near-fornix incision group(group C, 66 cases, 84 eyes). Based on the number of muscles involved in the operation, patients were divided into single extraocular muscle group(68 eyes), the double extraocular muscle group(106 eyes)and three extraocular muscle group(50 eyes). The children's eye surface health were evaluated. Other eye index such as lipidlayer thickness(LLT), tear meniscus height(TMH), corneal fluorescence staining score(CFSS), tear film break up time(TBUT), tear secretion(SⅠt), and meibomial gland opening were analyzed by Lipiview eye surface interferometer.RESULTS:The eye surface and meibomian gland function presented no significant difference in groups after surgery 1wk. Postoperative 1mo, ocular surface and muscle operating meibomian gland function were improved significantly(P