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Tytuł pozycji:

LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p

Tytuł:
LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p
Autorzy:
Zeng H
Wei Y
Wei X
Feng R
Temat:
linc00908
diffuse large b-cell lymphoma
mir-671-5p
proliferation
invasion
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło:
Cancer Management and Research, Vol Volume 13, Pp 3589-3599 (2021)
Wydawca:
Dove Medical Press, 2021.
Rok publikacji:
2021
Kolekcja:
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
1179-1322
Relacje:
https://www.dovepress.com/linc00908-promotes-diffuse-large-b-cell-lymphoma-development-by-down-r-peer-reviewed-fulltext-article-CMAR; https://doaj.org/toc/1179-1322
Dostęp URL:
https://doaj.org/article/22cd6696641b4e8d884831b2e1c65937  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.22cd6696641b4e8d884831b2e1c65937
Czasopismo naukowe
Hong Zeng, Yongqiang Wei, Xiaolei Wei, Ru Feng Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, People’s Republic of ChinaCorrespondence: Ru FengDepartment of Hematology, Nanfang Hospital, Southern Medical University, 1838 Guanzghou Avenue, Guagzhou, Guangdong, 510515, People’s Republic of ChinaTel +86-13922865798Email rufeng2000@21cn.comIntroduction: Emerging evidence has revealed that long noncoding RNA (lncRNA) play important role in almost all kinds of human cancers. LINC00908 has been reported to be involved in the development of prostate cancer, colorectal cancer and gastric cancer which was functioned as an oncogene. However, the potential biology role and molecular mechanism of LINC00908 in diffuse large B-cell lymphoma are still unclear.Methods: LINC00908 and miR-671-5p expression were evaluated in DLBCL tissues and cell lines using RT-qPCR. CCK-8 and transwell assay were used to analyze the in vitro role of LINC00908 in DLBCL progression. The xenograft model was used to explore the in vivo role of LINC00908 in DLBCL growth. The physical interaction between LINC00908 and miR-671-5p was confirmed using bioinformatics analysis and a dual luciferase assay, RIP and RNA pull down.Results: The expression of LINC00908 was markedly up-regulated in diffuse large B-cell lymphoma tissues and cell lines, and the decreased expression of LINC00908 significantly inhibited diffuse large B-cell lymphoma cell proliferation and invasion. Then, we revealed that LINC00908 directly interacted with miR-671-5p, which was down-regulated in diffuse large B-cell lymphoma cells and highly expressed with LINC00908 knockdown. Moreover, luciferase reporter assays and RNA immunoprecipitation (RIP) assay further proved that miR-671-5p is a direct target of LINC00908 in diffuse large B-cell lymphoma cells. Rescue experiments were also performed, and we confirmed that LINC00908 acts as an oncogene role in diffuse large B-cell lymphoma through miR-671-5p. Finally, the influence of LINC00908 silence significantly inhibited diffuse large B-cell lymphoma growth in vivo.Conclusion: LINC00908 promotes malignancy of diffuse large B-cell lymphoma through regulating miR-671-5p.Keywords: LINC00908, diffuse large B-cell lymphoma, miR-671-5p, proliferation, invasion

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