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Tytuł pozycji:

Kelch 13-propeller polymorphisms in Plasmodium falciparum from Jazan region, southwest Saudi Arabia

Tytuł :
Kelch 13-propeller polymorphisms in Plasmodium falciparum from Jazan region, southwest Saudi Arabia
Autorzy :
Ommer Mohammed Dafalla
Mohammed Alzahrani
Ahmed Sahli
Mohammed Abdulla Al Helal
Mohammad Mohammad Alhazmi
Elsiddig Mohammed Noureldin
Waheed Sideeg Mohamed
Tajeldin Bashir Hamid
Aymen Awad Abdelhaleem
Yahya Ali Hobani
Ommar Ali Arif
Ibrahim Munagi Bokar
Abdulazai Mohammed Hakami
Zaki Manawar Eisa
Pokaż więcej
Temat :
Plasmodium falciparum
k13 polymorphism
Jazan
Saudi Arabia
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Źródło :
Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020)
Wydawca :
BMC, 2020.
Rok publikacji :
2020
Kolekcja :
LCC:Arctic medicine. Tropical medicine
LCC:Infectious and parasitic diseases
Typ dokumentu :
article
Opis pliku :
electronic resource
Język :
English
ISSN :
1475-2875
Relacje :
http://link.springer.com/article/10.1186/s12936-020-03467-3; https://doaj.org/toc/1475-2875
DOI :
10.1186/s12936-020-03467-3
Dostęp URL :
https://doaj.org/article/e28c7786d4a64a5a90c0dafb16b83f76
Numer akcesji :
edsdoj.28c7786d4a64a5a90c0dafb16b83f76
Czasopismo naukowe
Abstract Background Artemisinin-based combination therapy (ACT) is recommended at the initial phase for treatment of Plasmodium falciparum, to reduce morbidity and mortality in all countries where malaria is endemic. Polymorphism in portions of P. falciparum gene encoding kelch (K13)-propeller domains is associated with delayed parasite clearance after ACT. Of about 124 different non-synonymous mutations, 46 have been identified in Southeast Asia (SEA), 62 in sub-Saharan Africa (SSA) and 16 in both the regions. This is the first study designed to analyse the prevalence of polymorphism in the P. falciparum k13-propeller domain in the Jazan region of southwest Saudi Arabia, where malaria is endemic. Methods One-hundred and forty P. falciparum samples were collected from Jazan region of southwest Saudi Arabia at three different times: 20 samples in 2011, 40 samples in 2016 and 80 samples in 2020 after the implementation of ACT. Plasmodium falciparum kelch13 (k13) gene DNA was extracted, amplified, sequenced, and analysed using a basic local alignment search tool (BLAST). Results This study obtained 51 non-synonymous (NS) mutations in three time groups, divided as follows: 6 single nucleotide polymorphisms (SNPs) ‘11.8%’ in samples collected in 2011 only, 3 (5.9%) in 2011and 2016, 5 (9.8%) in 2011 and 2020, 5 (9.8%) in 2016 only, 8 (15.7%) in 2016 and 2020, 14 (27.5%) in 2020 and 10 (19.6%) in all the groups. The BLAST revealed that the 2011 isolates were genetically closer to African isolates (53.3%) than Asian ones (46.7%). Interestingly, this proportion changed completely in 2020, to become closer to Asian isolates (81.6%) than to African ones (18.4%). Conclusions Despite the diversity of the identified mutations in the k13-propeller gene, these data did not report widespread artemisinin-resistant polymorphisms in the Jazan region where these samples were collected. Such a process would be expected to increase frequencies of mutations associated with the resistance of ACT.
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